The Retro-X Tet-On 3G Inducible Expression System combines the efficiency of retroviral gene transfer with the power of our premium tetracycline-regulated gene expression technology. The retroviral vectors in this system can be used to establish a tightly controlled inducible gene expression system in a wide variety of primary cell cultures and immortalized cell lines.
How it Works
Doxycycline-controlled “transactivators” are fusion proteins that contain a DNA-binding TetR domain joined to three minimal transcription activation domains from VP16 (Tet-On 3G, Tet-On Advanced and Tet-Off Advanced proteins). With very high specificity, these transactivators recognize and bind to a Tetracycline Response Element (TRE) that is located in the PTRE3G promoter of pRetroX-TRE3G which includes your gene of interest. Binding of the transactivator to the TRE promoter results in strong activation of the downstream gene. Tet-On 3G is the premium 3rd generation version of the technology, with the tightest TRE promoter and most sensitive transactivator.
In Tet-On systems, gene expression is activated by the addition of Dox to the growth medium.
Ideal Retroviral Packaging
Retro-X Tet-On 3G and Retro-X Tet-Express systems are supplied with our Retro-X Universal Packaging System (Cat. No. 631530) which produces high titers of MMLV retrovirus and allows you to customize the tropism of your retrovirus to efficiently transduce any cell line or primary cell culture, and thus greatly expands the applications for these inducible systems.
Retro-X Q Vector Technology
The vectors in these systems feature Clontech’s Retro-X Q-Series technology, which:
- Drives high titer virus production in packaging cells
- Inactivates viral LTRs in target cells to prevent promoter interference
- Coexpresses the transactivator and the Neor marker as a single transcription unit from the pRetroX-Tet3G Vector, ensuring that a high frequency of stable clones express the transactivator