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Products >  Viral_Transduction >  Retroviral_Vector_Systems >  MSCV

MSCV Retrovirus, Murine Stem Cell Virus System

The MSCV (Murine Stem Cell Virus) Retroviral Expression System contains vectors that are optimized for introducing and expressing target genes in pluripotent cell lines, including murine or human hematopoietic, embryonic stem (ES), and embryonal carcinoma (EC) cells. They can also be used effectively with most mammalian cell lines. This highly efficient system is ideal for analyzing gene function in development, embryogenesis, or immune response, in both cell culture and transgenic assays.

Designed for Expression in Hematopoietic Cells and Embryonic Stem Cells

The MSCV System contains three vectors: pMSCVneo, pMSCVhyg, and pMSCVpuro. These vectors contain a specially designed long terminal repeat (LTR) from the murine stem cell PCMV virus. This LTR differs from the MMLV LTR by several point mutations and a deletion that together enhance transcriptional activation and prevent transcriptional suppression in ES and EC cells. As a result, the PCMV LTR drives high-level, constitutive expression of the target gene in stem cells or other mammalian cell lines.

Packaging Cell Line Included

The MSCV System includes the RetroPack PT67 Packaging Cell Line, which produces high-titer virus able to infect a broad range of mammalian host cells.
At-A-Glance Documents Images & Data Resources

Features

  • Efficient gene expression in hematopoietic, ES and EC cells
  • Choice of three selectable markers
  • Complete retroviral system includes a PT67 packaging cell line

Applications

  • Retroviral transduction of a broad range of cell types including embryonic stem cells, embryonic carcinoma cells and hematopoietic cells

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
Products
Cat. # Product Package Size Price License # of Units Select
634401 MSCV Retroviral Expression System Each Call License Statements *
 
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

 


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