The MSCV (Murine Stem Cell Virus) Retroviral Expression System contains vectors that are optimized for introducing and expressing target genes in pluripotent cell lines, including murine or human hematopoietic, embryonic stem (ES), and embryonal carcinoma (EC) cells. They can also be used effectively with most mammalian cell lines. This highly efficient system is ideal for analyzing gene function in development, embryogenesis, or immune response, in both cell culture and transgenic assays.
Designed for Expression in Hematopoietic Cells and Embryonic Stem Cells
The MSCV System contains three vectors: pMSCVneo, pMSCVhyg, and pMSCVpuro. These vectors contain a specially designed long terminal repeat (LTR) from the murine stem cell PCMV virus. This LTR differs from the MMLV LTR by several point mutations and a deletion that together enhance transcriptional activation and prevent transcriptional suppression in ES and EC cells. As a result, the PCMV LTR drives high-level, constitutive expression of the target gene in stem cells or other mammalian cell lines.
Packaging Cell Line Included
The MSCV System includes the RetroPack PT67 Packaging Cell Line, which produces high-titer virus able to infect a broad range of mammalian host cells.