These retroviral vectors allow you to deliver and stably express your gene of interest and a fluorescent protein in a broad range of dividing cells. Depending on which vector you choose, the fluorescent protein and protein of interest can be:
- Fused together for subcellular localization or tag studies
- Simultaneously coexpressed from the same transcript but as separate proteins (IRES vectors)
Monomeric Proteins for Fusion Tags
DsRed-Monomer, AcGFP1, and mCherry proteins are truly monomeric fluorescent proteins that are ideal for use as fusion tags. mCherry is a very popular mutant derived by directed mutagenesis of mRFP1, a monomeric mutant of DsRed.
Super Bright Multimers for Reporter Studies
ZsGreen1 and tdTomato are supremely bright fluorescent proteins that are excellent reporters for gene expression, or for measuring transduction/transfection efficiency. They are 2.5X and 3.5X brighter than EGFP, respectively.
Stem Cell Applications
DsRed-Express2 is a rapidly maturing variant of Discosoma sp. red fluorescent protein (DsRed). It is highly soluble and was specially designed for stem cell applications.
