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Viral Transduction


Products >  Viral_Transduction >  Retroviral_Transduction_Tools >  Retrovirus_Titration

Retrovirus Titration by qRT-PCR

The Retro-X qRT-PCR Titration Kit provides a fast and simple method for measuring retrovirus titer. The kits use a quick RNA purification step before quantifying the number of retoviral genome copies using qRT-PCR and SYBR technologies.

Rapid Determination of Retroviral Titer

Whereas traditional titration methods require up to 10 days to complete, these titration kits require only 4 hours and work with all MMLV-based retroviral vectors (not MSCV). Using qRT-PCR dramatically shortens the time interval between viral harvest and target cell infection, allowing you to perform both on the same day.

Achieve Consistent Retroviral Transductions

A major advantage of retroviral gene delivery systems is the ability to control the level of gene expression by adjusting the multiplicity of infection (MOI) to dictate the number of viral genomes that enter your target cells. These titration kits allow you to make a rapid correlation between the retroviral copy number and retroviral infectivity in your cell type, and ultimately allow you to control the expression level between experiments.

How It Works

The qRT-PCR procedure entails subjecting serial dilutions of your sample and a control RNA of known copy number (provided) to one-step qRT-PCR. The retroviral copy number contained in your sample can then be determined by comparing its Ct value to the standard curve.

RNA purification and DNase I treatment steps are critical to the protocol so even viruses packaged using transient transfections, which always contain residual plasmid DNA, can be accurately quantified using this kit.

The kit includes sufficient material for 200 qRT-PCR reactions.

At-A-Glance Documents Images & Data Resources

Features

  • Confirm the success of your retroviral packaging reaction, to avoid wasting time with your experiments
  • Perform consistent experiments
  • Ensure sufficient MOIs so that 100% of your cells are transduced
  • Control copy number, and hence expression levels
  • 200 qRT-PCR Reactions are included
  • Purify 10 different virus preps

Applications

  • Retroviral transduction

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Package Size Price License # of Units Select
631453 Retro-X™ qRT-PCR Titration Kit 200 Rxns $396.00 License Statements
 

 

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