The Retro-X Universal Packaging System is our premium retroviral packaging system featuring the GP2-293 packaging cell line. All four commonly used envelopes are supplied on separate vectors including VSV-G, eco, ampho and 10A1 to allow you to choose the tropism that is most appropriate for your target cells. The envelope vector is cotransfected with your retroviral expression vector, and high titers of amphotropic, ecotropic, dualtropic or pantropic virions can be obtained in less than 48 hours.
The Retro-X System includes the Retro-X Vector Set and the RetroPack PT67 cell line. The RetroPack PT67 cell line is an NIH/3T3-based packaging cell line expressing the 10A1 viral envelope protein and is the recommended packaging cell line for creating a stable producer cell line for continuous production of retrovirus. If you wish to make retrovirus via transient transfection we recommend the Retro-X Universal Packaging System. Virus packaged from PT67 cells can be used to infect a broad range of mammalian cells including human mouse and rat cell types. The virus is able to enter cells via two different surface molecules, the amphotropic retrovirus receptor (Ram-1) and the GALV receptor.
Pantropic Retroviral Expression System
The Pantropic Retroviral Expression System, also features the GP2-293 packaging cell line that expresses gamma-retroviral gag and pol proteins together with the pVSV-G vector. Upon transient transfection of pVSV-G the cell line expresses an envelope glycoprotein from the vesicular stomatitis virus that is not dependent on a cell surface receptor; instead, it mediates viral entry through lipid binding and plasma membrane fusion. Two transfer expression vectors are included with the Pantropic System, giving you the choice of using a retroviral 5' LTR or the Drosophila hsp70 promoter to express your gene of interest.
AmphoPack 293 Cell Line
AmphoPack 293 can be used to produce recombinant MMLV-based viral particles that infect a broad range of mammalian cells including mouse, rat and human cells. AmphoPack 293 is derived from an HEK 293-based cell line that is easily transfected, and produces high viral titers. The viral envelope protein recognizes the amphotropic receptor (Ram-1).
All retroviral packaging cell lines and systems are compatible with all retroviral vectors sold by Clontech, including MMLV- and MSCV-based retrovirus.