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Viral Transduction


Products >  Viral_Transduction >  Lentiviral_Vector_Systems >  shRNA

Lentiviral shRNA Vector Systems (for RNAi)

The Lenti-X shRNA Expression System uses lentiviral shRNA vectors to deliver short hairpin RNA expression constructs into cells for the purpose of suppressing specific genes via RNA interference (RNAi).

  • The pLVX-shRNA2 Vector (available separately as Cat. No. 632179) provides shRNA and fluorescent ZsGreen1 coexpression to allow easy identification and sorting of transduced cells.

Lenti-X HTX Packaging System is Included

The Lenti-X shRNA Expression System includes our Lenti-X HTX Packaging System, which uses a novel, Tet-Off-based, 4th-generation packaging strategy to produce lentiviral supernatants with titers that are superior to most commercially available packaging systems. You can often transduce an entire dish of target cells with just 10–100 µl of unconcentrated viral supernatant.

High Titers Maximize Gene Knockdown

Given the varied efficacy of different shRNAs, high titers are essential to achieving the highest possible knockdown efficiency because they enable you to:

  • Transduce 100% of your target cell population.
  • Transduce every cell with sufficient copies for high-level shRNA expression.

At-A-Glance Documents Images & Data Resources

Features

  • Lentiviral vectors for high efficiency delivery of shRNA to most cell types
  • Highest lentiviral titers
  • Available in both selectable (puromycin) and fluorescent (ZsGreen1) formats

Applications

  • High efficiency gene suppression using shRNA expressed from a U6 promoter
  • shRNA delivery into hard-to-transfect cells using lentiviral shRNA vectors

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Package Size Price License # of Units Select
632177 Lenti-X™ shRNA Expression System Each $1,096.00 License Statements
632179 pLVX-shRNA2 Vector 10 ug $676.00 License Statements *
 
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

 

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