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Viral Transduction

Products >  Viral_Transduction >  Lentiviral_Vector_Systems >  shRNA

Lentiviral shRNA Vector Systems (for RNAi)

The Lenti-X shRNA Expression System uses lentiviral shRNA vectors to deliver short hairpin RNA expression constructs into cells for the purpose of suppressing specific genes via RNA interference (RNAi). The Lenti-X shRNA Expression System includes pLVX-shRNA1 (with puromycin selection) and our Lenti-X Packaging Single Shots (VSV-G), a 4th-generation packaging system used to produce lentiviral supernatants with titers that are superior to most commercially available packaging systems. Lenti-X Packaging Single Shots are pre-aliquoted, lyophilized, single tubes that contain Xfect Transfection Reagent premixed with an optimized formulation of VSV-G pseudotyped Lenti-X lentiviral packaging plasmids. The pLVX-shRNA2 Vector (available separately as Cat. No. 632179) provides shRNA and fluorescent ZsGreen1 coexpression to allow easy identification and sorting of transduced cells.

High Titers Maximize Gene Knockdown

Given the varied efficacy of different shRNAs, high titers are essential to achieving the highest possible knockdown efficiency because they enable you to:

  • Transduce 100% of your target cell population.
  • Transduce every cell with sufficient copies for high-level shRNA expression.

  At-A-Glance   Documents   Images & Data   Resources



  • High efficiency gene suppression using shRNA expressed from a U6 promoter
  • shRNA delivery into hard-to-transfect cells using lentiviral shRNA vectors

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Cat. # Product Contents Size Price License Units Select
632177 Lenti-X™ shRNA Expression System Each $1,096.00 License Statements
632179 pLVX-shRNA2 Vector 10 ug $676.00 License Statements *
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.


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