The Lenti-X shRNA Expression System uses lentiviral shRNA vectors to deliver short hairpin RNA expression constructs into cells for the purpose of suppressing specific genes via RNA interference (RNAi). The Lenti-X shRNA Expression System includes pLVX-shRNA1 (with puromycin selection) and our Lenti-X Packaging Single Shots (VSV-G), a 4th-generation packaging system used to produce lentiviral supernatants with titers that are superior to most commercially available packaging systems. Lenti-X Packaging Single Shots are pre-aliquoted, lyophilized, single tubes that contain Xfect Transfection Reagent premixed with an optimized formulation of VSV-G pseudotyped Lenti-X lentiviral packaging plasmids. The pLVX-shRNA2 Vector (available separately as Cat. No. 632179) provides shRNA and fluorescent ZsGreen1 coexpression to allow easy identification and sorting of transduced cells.
High Titers Maximize Gene Knockdown
Given the varied efficacy of different shRNAs, high titers are essential to achieving the highest possible knockdown efficiency because they enable you to:
- Transduce 100% of your target cell population.
- Transduce every cell with sufficient copies for high-level shRNA expression.