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Products >  Viral_Transduction >  Lentiviral_Vector_Systems >  Tet-Inducible

Lentiviral Tet-One Inducible Expression Systems

The Tet-One System allows you to generate inducible expression systems as simply as constitutive expression systems. Previous inducible expression systems required transduction of two separate vectors into your cells. With the Tet-One system, both the doxycycline-responsive transactivator and your gene of interest are combined onto the same vector. This allows for greater transduction efficiency, while maintaining the low background expression and up to 25,000-fold induction (Heinz et al. 2011) in response to doxycycline that is found in the Tet-On 3G systems.

Efficient Lentivial Packaging System

Lenti-X Packaging Single Shots (VSV-G), included with each Tet-One kit, generate high lentiviral titers without the need to concentrate virus. The lentivirus generated is useful for transduction of broad range of cell types, including hard-to-transfect cell lines. This allows you to easily transduce cells with a minimum of viral supernatant. Monitoring of viral titers is made quick and easy with the included Lenti-X GoStix.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • Highly efficient Lenti-X Packaging Single Shots (VSV-G) (included) generate high titers with a very high safety profile
  • Tightly control expression of your gene of interest with the TRE3G promoter
  • Simple, single vector design
  • Option to use a puromycin resistance cassette to select for stable clones
  • Tet-Express Systems and Tet-On 3G lentiviral systems are also available

Applications

  • Tetracycline-inducible expression in the widest range of hard-to-transfect cell types

References

Heinz, N., Schambach, A., Galla, M., Maetzig, T., Baum, C., Loew R., & Schiedlmeier, B. (2011) Retroviral and transposon-based Tet-regulated all-in-one vectors with reduced background expression and improved dynamic range. Human Gene Ther. 22(2):166–176.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 

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