Optimized Lentiviral Vectors and Expression Systems
Clontech offers a variety of highly optimized lentiviral vectors for many applications. These lentiviral vectors are key players in all of Clontech’s highly effective Lenti-X gene delivery systems, which can be used with almost any mammalian cell type, including dividing and nondividing cells, primary cell cultures, stem cells, and neurons. All of our pLVX lentiviral vectors contain HIV-1 LTRs and the lentiviral packaging signal (Ψ), and we’ve included specific elements to improve transgene expression, virus titer, and overall lentiviral vector function:
- WPRE: A woodchuck hepatitis virus posttranscriptional regulatory element prevents poly A site readthrough, promotes RNA processing and maturation, and increases nuclear export of RNA. In genomic transcripts, it enhances vector packaging and increases titer. In transduced target cells, the WPRE boosts transgene expression by facilitating mRNA transcript maturation.
- cPPT/CTS: A central polypurine tract/central termination sequence creates a “DNA flap” that increases nuclear importation of the viral genome during target cell infection. The cPPT/CTS element improves vector integration and transduction efficiency.
- RRE: A Rev response element increases titers by promoting the nuclear export of unspliced viral genomic RNA.
Use these lentiviral vectors with any of our highly advanced, safe, and easy-to-use 4th-generation Lenti-X HTX Packaging Systems, which produce high titers of VSV-G- or ecotropic-pseudotyped lentivirus.
To learn more about lentiviral vector safety, download our Lentiviral Vector Safety flyer.
|
Lentiviral Vector Core Elements
|
|
|
Express your gene of interest from a strong CMV promoter, and select for lentiviral integration using antibiotic selection.
|
|
|
Co-express your gene of interest and an antibiotic or fluorescent selectable marker from the same transcript using these IRES-containing lentiviral vector systems.
|
|
|
Express your gene of interest from an EF-1 alpha promoter in cell types where the CMV promoter can be silenced over time, such as hematopoietic or stem cells.
|
|
|
Obtain tightly controlled, inducible expression clones of your gene of interest with only a single vector.
|
|
|
Clone your shRNA downstream of a U6 promoter to knock down gene expression in hard to transfect cells.
|
|
|
Clone your gene downstream of a ProteoTuner destabilization domain (DD) and control the stability of your protein with Shield1, a small molecule ligand, which protects the DD fusion protein from being degraded.
|
|
|
Clone your gene in-frame with one of Clontech’s many fluorescent proteins (FP) and monitor the expression and subcellular localization of your protein of interest.
|
|
|
Monitor activity of your favorite promoter using a reporter system that expresses bright fluorescent proteins or secreted luciferase.
|
|
|
Monitor activity of your favorite promoter using a reporter system that expresses a cyan, green or red fluorescent protein on demand.
|
