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Products >  Viral_Transduction >  Lentiviral_Vector_Systems >  IRES_Bicistronic

Bicistronic Lentiviral Vector Systems

Lenti-X Bicistronic Lentiviral Vectors and Expression Systems allow you to deliver and stably express your gene of interest, along with a coexpressed bright fluorescent protein or an antibiotic selection marker, in virtually any mammalian cell type. The coexpressed proteins are produced from a single mRNA transcript, so you can be assured that your protein of interest is present in cells in which the detectable marker is also present. The Lenti-X Bicistronic Expression System is available in three different formats with expression vectors containing Neor, Hygr, or Puror markers.

Alternatively, you can coexpress your gene of interest with Clontech’s Living Colors mCherry, tdTomato, and ZsGreen1 fluorescent proteins using the pLVX-IRES-mCherry, pLVX-IRES-tdTomato, and pLVX-IRES-ZsGreen1 Vectors. These are supremely bright fluorescent proteins that are excellent reporters for gene expression or for measuring the efficiency of transduction or transfection. mCherry is a very popular mutant derived by directed mutagenesis of mRFP1, a monomeric mutant of DsRed.

Simultaneous Expression of Two Proteins—How Does it Work?

The key element of these bicistronic lentiviral vectors is an optimized internal ribosome entry site (IRES) that permits your gene of interest and a second detectable protein to be coexpressed from a single mRNA transcript. While translation initiation of eukaryotic mRNAs occurs almost exclusively at the 5' cap, the IRES lures ribosomes to begin translation at a second, internal location. The result is that two proteins are expressed simultaneously from a single bicistronic mRNA transcript.

The Lenti-X HTX Packaging System & Lenti-X 293T Cell Line

We highly recommended these two products for the production of lentivirus from Lenti-X lentiviral vectors. The Lenti-X HTX Packaging System, together with the Lenti-X 293T Cell Line, generates titers that are much higher than those produced by other commercially available systems. Frequently, an entire dish of target cells can be effectively transduced using just 10 µl of unconcentrated viral supernatant.

At-A-Glance Documents Images & Data Resources

Features

  • Track expression of your protein using fluorescence microscopy
  • Expression of your protein is assured in antibiotic-resistant clones
  • Bright fluorescent protein coexpression

Applications

  • Monitor the expression of your lentiviral-delivered genes with fluorescent tags and antibiotic selection markers.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
Products
Cat. # Product Package Size Price License # of Units Select
632182 Lenti-X™ Bicistronic Expression System (Hyg) Each $1,096.00 License Statements *
632181 Lenti-X™ Bicistronic Expression System (Neo) Each $1,096.00 License Statements *
632183 Lenti-X™ Bicistronic Expression System (Puro) Each $1,096.00 License Statements *
631253 Lenti-X™ Expression System (EF1alpha Version) Each $1,096.00 License Statements *
631982 pLVX-EF1alpha-IRES-ZsGreen1 Vector 10 ug $676.00 License Statements *
631987 pLVX-EF1alpha-IRES-mCherry Vector 10 ug $676.00 License Statements *
632187 pLVX-IRES-ZsGreen1 Vector 10 ug $676.00 License Statements *
631237 pLVX-IRES-mCherry Vector 20 ug $676.00 License Statements *
631238 pLVX-IRES-tdTomato Vector 20 ug $676.00 License Statements *
 
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

 

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