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Viral Transduction


Products >  Viral_Transduction >  Lentiviral_Vector_Systems >  Fluorescent_Protein

Lentiviral Vectors with Fluorescent Proteins

These lentiviral vectors allow you to deliver and stably express your gene of interest as a fluorescent fusion protein in virtually any cell type, including dividing and nondividing cells, stem cells, terminally differentiated cells, and neuronal cells. Three bicistronic lentiviral vectors (pLVX-IRES-mCherry, pLVX-IRES-tdTomato, and pLVX-IRES-ZsGreen1) are also available to allow your gene of interest and a bright fluorescent protein to be coexpressed from a single mRNA transcript.

Clontech’s Living Colors DsRed-Monomer, AcGFP1, and mCherry proteins are truly monomeric fluorescent proteins that are ideal for use as fusion tags. ZsGreen1 and tdTomato are supremely bright fluorescent proteins that are excellent reporters for gene expression, or for measuring transduction/transfection efficiency. mCherry is a very popular mutant derived by directed mutagenesis of mRFP1, a monomeric mutant of DsRed. DsRed-Express2 is a rapidly maturing variant of Discosoma sp. red fluorescent protein (DsRed). It is highly soluble and was designed to be better suited for cell and stem cell applications.

All Living Colors proteins are detectable without added cofactors or substrates, making them exceptional, noninvasive tools for live cell studies.

DsRed-Monomer & AcGFP1

  • Truly monomeric proteins (see DsRed-Monomer for more information)
  • Ideal for tagging proteins for trafficking and localization studies
  • Distinct spectra make them excellent choices for double labeling experiments

ZsGreen1 & tdTomato

  • The brightest green and red fluorescent proteins—(2.5X and 3.5X brighter than EGFP, respectively)
  • IRES bicistronic expression for monitoring transduction efficiency
  • Separate expression of your cDNA and a fluorescent protein from a single transcript

mCherry

  • A bright monomeric protein
  • Matures very rapidly—its t1/2 for maturation at 37°C is approximately 15 minutes

DsRed-Express2

  • Rapidly maturing red fluorescent protein with enhanced solubility and reduced green emission
  • Well-suited for cell and stem cell applications

The Lenti-X HTX Packaging System & Lenti-X 293T Cell Line

We highly recommended these two products for the production of high-titer lentivirus from all our lentiviral vectors. The Lenti-X HTX Packaging System, together with the Lenti-X 293T Cell Line, generates titers that are much higher than those produced by other commercially available systems. Frequently, an entire dish of target cells can be effectively transduced using just 10 µl of unconcentrated viral supernatant.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • Visualize your protein using fluorescence microscopy
  • Generate both N- & C-terminal fusions to bright fluorescent proteins
  • Truly monomeric red and green tags

Applications

  • Monitor the expression of your lentiviral-delivered genes with fluorescent tags and markers.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
632155 null 10 ug $676.00 License Statements *
632154 null 10 ug $676.00 License Statements *
632557 null 10 ug $676.00 License Statements *
632558 null 10 ug $676.00 License Statements *
632559 null 10 ug $676.00 License Statements *
632560 null 10 ug $676.00 License Statements *
632153 null 10 ug $676.00 License Statements *
632152 null 10 ug $676.00 License Statements *
632187 null 10 ug $676.00 License Statements *
631237 null 20 ug $676.00 License Statements *
631238 null 20 ug $676.00 License Statements *
632587 null 10 ug $676.00 License Statements *
632586 null 10 ug $676.00 License Statements *
632566 null 10 ug $676.00 License Statements *
632565 null 10 ug $676.00 License Statements *
632561 null 10 ug $676.00 License Statements *
632562 null 10 ug $676.00 License Statements *
632564 null 10 ug $676.00 License Statements *
632563 null 10 ug $676.00 License Statements *
 
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

 

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631247 Lenti-X™ HTX Packaging System 20 Rxns $876.00
 
 
 

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