Lentiviral Vector Systems with an EF-1 Alpha Promoter
Lenti-X lentiviral vector systems with EF-1 alpha promoters allow you to achieve robust, constitutive, long-term expression of your gene of interest in cell types in which CMV promoters are often silenced, such as hematopoietic and stem cells. The EF-1 alpha promoter, which offers a broad host range, is derived from the human EEF1A1 gene that expresses the alpha subunit of eukaryotic elongation factor 1 (1, 2).
Expression Options of Lentiviral Vectors with an EF-1 Alpha Promoter
The Lenti-X Expression System (EF1a Version) is a complete system that includes the pLVX-EF1a-IRES-Puro Vector, and our 4th Generation Lentiviral Packaging System. Alternatively, you can express your protein fused to a red (mCherry or DsRed-Monomer) or green (AcGFP1) fluorescent protein, or express your protein and a fluorescent protein separately from an internal ribosome entry site (IRES).