Home Takara Clontech Logo Takara Clontech
login | register | order now | view cart | feedback
Products
Viral Transduction


Products >  Viral_Transduction >  Lentiviral_Vector_Systems >  EF-1_Alpha_Promoter

Lentiviral Vector Systems with an EF-1 Alpha Promoter

Lenti-X lentiviral vector systems with EF-1 alpha promoters allow you to achieve robust, constitutive, long-term expression of your gene of interest in cell types in which CMV promoters are often silenced, such as hematopoietic and stem cells. The EF-1 alpha promoter, which offers a broad host range, is derived from the human EEF1A1 gene that expresses the alpha subunit of eukaryotic elongation factor 1 (1, 2).

Expression Options of Lentiviral Vectors with an EF-1 Alpha Promoter

The Lenti-X Expression System (EF1a Version) is a complete system that includes the pLVX-EF1a-IRES-Puro Vector, and our 4th Generation Lentiviral Packaging System. Alternatively, you can express your protein fused to a red (mCherry or DsRed-Monomer) or green (AcGFP1) fluorescent protein, or express your protein and a fluorescent protein separately from an internal ribosome entry site (IRES).

  At-A-Glance   Documents   Images & Data   Resources

Features

  • High-titer lentiviral vector system
  • Constitutive expression
  • Robust, long-term expression, in the broadest range of cell type
  • Bright fluorescent proteins to monitor expression or localization

Applications

  • If CMV promoters are silenced in your cell type, try EF-1 alpha
  • Lentiviral transduction of stem cells
  • Lentiviral transduction of hematopoietic cells

References

  1. Uetsuki, T., Naito, A., Nagata, S., and Kaziro, Y. (1989). Isolation and Characterization of the Human Chromosomal Gene for Polypeptide Chain Elongation Factor-1. J. Biol. Chem. 264:5791–5798.
  2. Kim, D., Uetsuki, T., Kaziro, Y., Yamaguchi, N. and Sugano, S. (1990). Use of the human elongation factor 1a promoter as a versatile and efficient expression system. Gene 91:217–223.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
631253 Lenti-X™ Expression System (EF1alpha Version) Each $1,096.00 License Statements
631984 pLVX-EF1alpha-AcGFP1-C1 Vector 10 ug $676.00 License Statements *
631983 pLVX-EF1alpha-AcGFP1-N1 Vector 10 ug $676.00 License Statements *
631989 pLVX-EF1alpha-DsRed-Monomer-C1 Vector 10 ug $676.00 License Statements *
631990 pLVX-EF1alpha-DsRed-Monomer-N1 Vector 10 ug $676.00 License Statements *
631982 pLVX-EF1alpha-IRES-ZsGreen1 Vector 10 ug $676.00 License Statements *
631987 pLVX-EF1alpha-IRES-mCherry Vector 10 ug $676.00 License Statements *
631985 pLVX-EF1alpha-mCherry-C1 Vector 10 ug $676.00 Application Error
 
Application Error
 

The application is currently not available or an unexpected error has occured. Please try again later.
If this message persists, please contact the system administrator.

 

 
Application Error
 
Application Error
 

The application is currently not available or an unexpected error has occured. Please try again later.
If this message persists, please contact the system administrator.

 

 

Application Error
 
Application Error
 

The application is currently not available or an unexpected error has occured. Please try again later.
If this message persists, please contact the system administrator.