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Viral Transduction


Products >  Viral_Transduction >  Lentiviral_Vector_Systems >  Cell_Surface_Marker

Selection Using a Cell Surface Marker

CherryPicker is a red fluorescent cell surface marker that is expressed when you transduce your cells using the Lenti-X CherryPicker Cell Capture Systems. Upon transduction, the CherryPicker protein is expressed, transported, and anchored to the plasma membrane where it is exposed extracellularly. Selecting positively transduced cells is fast and simple using magnetic beads that bind specifically to the CherryPicker marker.

Selection with CherryPicker (rather than antibiotic selection) means that you do not have to wait for results or expose sensitive cells to harsh treatments. The system has the added benefit that you can also see the positively transduced cells under a fluorescent microscope. Systems are available to select for transgene expression or for promoter activation.

Kit Components

Lenti-X CherryPicker Cell Capture System (IRES), Cat. No. 632575
Use this kit to select cells expressing a protein of interest

  • Bicistronic lentiviral CherryPicker vector
  • Lentiviral control vector
  • CherryPicker Assay Kit (also sold separately)
    • CherryPicker Reagent Kit
    • Magnetic beads

Lenti-X CherryPicker Cell Capture System, Cat. No. 632574
Use this kit to select cells where a promoter of interest is active

  • Promoterless lentiviral CherryPicker vector
  • Lentiviral control vector
  • CherryPicker Assay Kit (also sold separately)
    • CherryPicker Reagent Kit
    • Magnetic beads

At-A-Glance Documents Images & Data Resources

Features

Learn more about CherryPicker

  • Rapid selection of transduced cells using a fluorescent cell surface marker
  • No need to wait for drug selection
  • Simple selection of transduced cells using magnetic beads
  • Visualize transduced cells because the cell surface marker is fluorescent
  • Select for co-expression of your transgene using the IRES version
  • Select for your promoter activity using the non-IRES version

Applications

  • Transduction of cells using high titer lentivirus
  • Selection of positively transduced cells using a fluorescent cell surface marker

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Package Size Price License # of Units Select
632570 CherryPicker™ Assay Kit 60 Rxns $448.00 License Statements
632571 CherryPicker™ Assay Kit 120 Rxns $865.00 License Statements
632574 Lenti-X™ CherryPicker™ Cell Capture System 60 Rxns $755.00 License Statements *
632575 Lenti-X™ CherryPicker™ Cell Capture System (IRES) 60 Rxns $755.00 License Statements *
 
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

 

Required Products
Cat. # Product Package Size Price # of Units Select
631964 Magnetic Stand 2 x 1.5 mL $42.00
 

 

 

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