The Lenti-X p24 Rapid Titer Kit is designed for the rapid determination of lentiviral supernatant titers by p24 ELISA.
What is p24?
Recombinant lentiviruses generated for research use contain major structural proteins including the envelope proteins, matrix proteins, and virus core proteins. The gag gene encodes the viral capsid protein (p24); the nucleocapsid proteins (p6 and p7); and a matrix protein (p17) (see Images & Data tab). Clontech’s Lenti-X p24 Rapid Titer Kit specifically measures the amount of p24 capsid protein present in your viral supernatant. The level of p24 correlates directly with virus titer.
How Does a p24 ELISA Work?
The virus in your supernatant is first lysed in the supplied buffer system to release p24. The sample is then applied to 96-well microtitration plate (made up of 12 separable 8-well strips), in which the wells are pre-coated with murine anti-p24 capture antibody. After washing to remove unbound lysate, the amount of specifically bound p24 is then detected using a biotinylated anti-p24 secondary antibody, streptavidin-HRP (horseradish peroxidase), and a color development reagent. The kit is supplied with a p24 control which is used to generate a standard curve and calibrate the p24 equivalent of your supernatant, and its titer.
