Clontech
login | register | order now | view cart | feedback
Products
Viral Transduction


Products >  Viral_Transduction >  Lentiviral_Transduction_Tools >  Lentivirus_Titration >  p24_ELISA

Lentivirus Titration by p24 ELISA

The Lenti-X p24 Rapid Titer Kit is designed for the rapid determination of lentiviral supernatant titers by p24 ELISA.

What is p24?

Recombinant lentiviruses generated for research use contain major structural proteins including the envelope proteins, matrix proteins, and virus core proteins. The gag gene encodes the viral capsid protein (p24); the nucleocapsid proteins (p6 and p7); and a matrix protein (p17) (see Images & Data tab). Clontech’s Lenti-X p24 Rapid Titer Kit specifically measures the amount of p24 capsid protein present in your viral supernatant. The level of p24 correlates directly with virus titer.

How Does a p24 ELISA Work?

The virus in your supernatant is first lysed in the supplied buffer system to release p24. The sample is then applied to 96-well microtitration plate (made up of 12 separable 8-well strips), in which the wells are pre-coated with murine anti-p24 capture antibody. After washing to remove unbound lysate, the amount of specifically bound p24 is then detected using a biotinylated anti-p24 secondary antibody, streptavidin-HRP (horseradish peroxidase), and a color development reagent. The kit is supplied with a p24 control which is used to generate a standard curve and calibrate the p24 equivalent of your supernatant, and its titer.

At-A-Glance Documents Images & Data Resources

Features

  • Rapidly determine the titer of your lentivirus prep using a p24 ELISA
  • Collect lentiviral supernatant, lyse, bind, wash, and detect
  • 96-well format (12 x 8-well strips)
  • p24 control included

Why You Need To Know Your Titer

  • Confirm the success of your packaging reaction to avoid wasting time with your experiments
  • Perform consistent experiments
  • Ensure sufficient MOI so that 100% of your cells are transduced
  • Control copy number and hence expression levels

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Package Size Price # of Units Select
632200 Lenti-X™ p24 Rapid Titer Kit 96 Rxns $469.00
 

 

Recommended Products
Cat. # Product Package Size Price License # of Units Select
T100A RetroNectin® Recombinant Human Fibronectin Fragment 0.5 mg $166.00 License Statements
631231 Lenti-X™ Concentrator 100 mL $194.00  
631243 Lenti-X™ GoStix™ 20 Tests $299.00 License Statements
631247 Lenti-X™ HTX Packaging System 20 Rxns $876.00 License Statements
 
 

Clontech is a Takara Bio Company © 2014 Privacy Policy | Terms & Conditions