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Viral Transduction


Products >  Viral_Transduction >  Lentiviral_Transduction_Tools >  Lentivirus_Concentration

Lentivirus Concentration—Concentrate Lentiviral Supernatants Without Ultracentrifugation

Lenti-X Concentrator provides a fast, simple, and highly efficient method for concentrating any lentiviral stock. In the simple protocol, you just mix your lentiviral supernatant with the lentivirus concentration reagent, incubate for a short period, and spin the mixture in a standard centrifuge.

100-Fold Increase in Titer

You’ll increase your lentiviral titer by up to 100-fold in ~1 hr, and obtain excellent recoveries—with no ultracentrifugation. Lenti-X Concentrator works for all lentiviral supernatants, including those made from any of Clontech’s Lenti-X Systems, and the procedure can be scaled up or down to best suit your needs.

Far Simpler Than Ultracentrifugation

Compared to ultracentrifugation, the Lenti-X Concentrator is more flexible in terms of scale, is easier to use than ultracentrifugation and requires no specialized equipment.

Scalable for Supernatants of Any Volume, Any Titer

The Lenti-X Concentrator reagent is a 4X concentrate, so it can be added to any volume of supernatant containing any amount of virus or any starting titer. At Clontech scalability has been successful for supernatant volumes up to 250 ml.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • Simple mix and spin protocol
  • Hassle-free and easily scaled up for large volumes
  • No ultracentrifugation required
  • 100X concentration
  • 90% yield

Applications

  • Lentiviral transduction studies
  • Increased multiplicity of infection (MOI)
  • Rescue of low titer supernatants

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Size Price Units Select
631231 Lenti-X™ Concentrator 100 mL $194.00
631232 Lenti-X™ Concentrator 500 mL $684.00
 

 

 
 

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