Lenti-X Concentrator provides a fast, simple, and highly efficient method for concentrating any lentiviral stock. In the simple protocol, you just mix your lentiviral supernatant with the lentivirus concentration reagent, incubate for a short period, and spin the mixture in a standard centrifuge.
100-Fold Increase in Titer
You’ll increase your lentiviral titer by up to 100-fold in ~1 hr, and obtain excellent recoveries—with no ultracentrifugation. Lenti-X Concentrator works for all lentiviral supernatants, including those made from any of Clontech’s Lenti-X Systems, and the procedure can be scaled up or down to best suit your needs.
Far Simpler Than Ultracentrifugation
Compared to ultracentrifugation, the Lenti-X Concentrator is more flexible in terms of scale, is easier to use than ultracentrifugation and requires no specialized equipment.
Scalable for Supernatants of Any Volume, Any Titer
The Lenti-X Concentrator reagent is a 4X concentrate, so it can be added to any volume of supernatant containing any amount of virus or any starting titer. At Clontech scalability has been successful for supernatant volumes up to 250 ml.