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Viral Transduction


Products >  Viral_Transduction >  Lentiviral_Particles >  iPS_Cell_Reprogramming

Ready-to-Use Lentiviral Particles for Human Somatic Cell Reprogramming

It has been demonstrated that differentiated cells can be reprogrammed to a pluripotent state. Takahashi and Yamanaka first showed that expression of just four transcription factors, Oct4, Sox2, Klf4 and c-Myc, could induce pluripotency in mouse (1) and human (2) fibroblasts. Yu and colleagues showed that expression of Oct4, Sox2, Nanog, and Lin28 was sufficient to reprogram human somatic cells to pluripotent stem cells (3). Somatic cell-derived reprogrammed cells have similar molecular properties as embryonic stem cells and are referred to as induced pluripotent stem (iPS) cells. Because iPS cells can self-renew and have the potential to differentiate into any cell type, iPS cells are potentially important tools in regenerative medicine and disease modelling. Clontech has partnered with Vectalys to provide high-titer, high-purity, ready-to-use lentiviral particles that allow delivery of these reprogramming factors to human somatic cells for generating iPS cells. These recombinant lentiviral particles contain genes for the reprogramming factors expressed either individually or as a polycistronic transcript. Expression of the Yamanaka factors can also be tightly controlled using Clontech’s Tet-On 3G Tetracycline Inducible Expression System (Cat. No. 0027VCT and 0028VCT).

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Features

  • Highly concentrated lentiviral particles; all lots are >109 transducing units (TU) per ml
  • What does 109 TU/ml mean?
    • In each microliter there will be at least 1 x 106 functional lentivirus particles.
    • Each kit (60 µl) is sufficient for 30 transductions (using an MOI of 10) in a 6-well plate containing 2 x 105 cells/well
  • Functional titer provided, not a just a particle count
  • High-purity lentiviral particles—transduce human somatic cells without cytotoxicity
  • >90% transduction efficiency can be obtained even at very low MOI
  • Choice of constitutive or inducible (Tet-On 3G system) control of reprogramming factor expression
  • Yamanaka factors provided on separate vectors or combined on a single vector for polycistronic expression (see map schematics)

Applications

  • Reprogramming human somatic cells into iPS cells






References

  1. Takahashi, K. and Yamanaka, S. (2006) Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126:663–676.
  2. Takahashi, K. and Yamanaka, S. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131:861–872.
  3. Yu, J., et al. (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318:1917–1920.

Additional Product Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.




 
 
Products
Cat. # Product Contents Size Price License Units Select
0001VCT rLV.EF1.OSKM-9 3 x 20 uL $1,717.00 License Statements
0002VCT rLV.EF1.Oct4-9 3 x 20 uL $1,717.00 License Statements
0003VCT rLV.EF1.Sox2-9 3 x 20 uL $1,717.00 License Statements
0004VCT rLV.EF1.Klf4-9 3 x 20 uL $1,717.00 License Statements
0005VCT rLV.EF1.Myc-9 3 x 20 uL $1,717.00 License Statements
0006VCT rLV.EF1.Lin28-9 3 x 20 uL $1,717.00 License Statements
0007VCT rLV.EF1.Nanog-9 3 x 20 uL $1,717.00 License Statements
0027VCT rLV.EF1.Tet3G-9 3 x 20 uL $1,717.00 License Statements *
0028VCT rLV.TRE3G.OSKM-9 3 x 20 uL $1,717.00 License Statements *
0055VCT rLV.EF1.Tet3G-9 1 x 20 uL $606.00 License Statements *
 
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