Clontech
login | register | order now | view cart | feedback
Products
Viral Transduction


Products >  Viral_Transduction >  Lentiviral_Particles >  Tet-On_3G

Tet-On 3G Transactivator Lentiviral Particles

Clontech has partnered with Vectalys to provide high titer, high purity, ready-to-use lentiviral particles that deliver the Tet-On 3G transactivator to target cells. Cells transduced with these particles will express the Tet-On 3G transactivator protein, allowing high level expression of any gene of interest (GOI) under the control of a TRE3G promoter (PTRE3G) in the presence of doxycycline (Dox), a tetracycline analog. Learn more about Tet-On 3G inducible gene expression.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • What does 109 TU/ml mean?
    • In each microliter there will be at least 1 x 106 functional lentivirus particles.
    • Each 60 µl kit is sufficient for 30 transductions (using an MOI of 10) in a 6-well plate containing 2 x 105 cells/well
  • Gold standard tetracycline transactivator in a ready-to-use lentivirus format
  • Premium quality; produced via a multi-step purification process which results in very high titers and purity
    • Very high titer; functional titer of >1 x 109 transduction units per ml (TU/ml)
    • Functional titer provided, not a just a particle count
    • High purity - transduce target cells without cytotoxicity
    • >90% transduction efficiency can be obtained even at very low M.O.I.
  • Can be combined with any vector containing a TRE promoter to obtain tetracycline-inducible expression of your gene of interest (we recommend the TRE3G promoter)
  • Learn more about Tet-On 3G Technology

Applications

  • Tightly controlled, inducible expression of your gene of interest in hard-to-transfect mammalian cells.








Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.




 
Products
Cat. # Product Contents Size Price License Units Select
0027VCT rLV.EF1.Tet3G-9 3 x 20 uL $1,717.00 License Statements *
0055VCT rLV.EF1.Tet3G-9 1 x 20 uL $606.00 License Statements *
 
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

 

 

Takara Bio USA is a Takara Bio Company © 2017 Privacy Policy | Terms & Conditions | Terms of Use