Lenti-X Packaging Single Shots are a fourth-generation lentiviral packaging system that provides an extremely simple and consistent one-step method for producing high-titer lentivirus. No additional transfection reagent is needed because Lenti-X Packaging Single Shots consist of pre-aliquoted, lyophilized, single tubes of Xfect Transfection Reagent premixed with an optimized formulation of Lenti-X lentiviral packaging plasmids. High-titer virus is produced by simply reconstituting this mixture with your lentiviral vector of choice in sterile water and adding it to 293T cells, e.g., Lenti-X 293T Cells (Cat. No. 632180), in a 10-cm dish. Choose from three types of Lenti-X Packaging Single Shots optimized for different tropisms and integration properties:
- Lenti-X Packaging Single Shots (VSV-G) provide the highest titers (107–108 IFU per ml) and widest tropism
- Lenti-X Packaging Single Shots (Ecotropic) restrict tropism to cells of mouse and rat origin
- Lenti-X Packaging Single Shots (Integrase Deficient) produce VSV-G pseudotyped lentivirus that has a significantly reduced ability to integrate
The amount of reagent and vector in each tube is optimized for lentivirus production in a 10-cm dish. Transfections can be carried out entirely in the presence of serum. Use of tetracycline-free FBS is critical for achieving high titers with this technology.