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Viral Transduction

Products >  Viral_Transduction >  Lentiviral_Packaging >  Lenti-X_Packaging_Single_Shots

High-Titer, One-Step Lentivirus Production—Lenti-X Packaging Single Shots

Lenti-X Packaging Single Shots are a fourth-generation lentiviral packaging system that provides an extremely simple and consistent one-step method for producing high-titer lentivirus. No additional transfection reagent is needed because Lenti-X Packaging Single Shots consist of pre-aliquoted, lyophilized, single tubes of Xfect Transfection Reagent premixed with an optimized formulation of Lenti-X lentiviral packaging plasmids. High-titer virus is produced by simply reconstituting this mixture with your lentiviral vector of choice in sterile water and adding it to 293T cells, e.g., Lenti-X 293T Cells (Cat. No. 632180), in a 10-cm dish. Choose from three types of Lenti-X Packaging Single Shots optimized for different tropisms and integration properties:

  • Lenti-X Packaging Single Shots (VSV-G) provide the highest titers (107–108 IFU per ml) and widest tropism
  • Lenti-X Packaging Single Shots (Ecotropic) restrict tropism to cells of mouse and rat origin
  • Lenti-X Packaging Single Shots (Integrase Deficient) produce VSV-G pseudotyped lentivirus that has a significantly reduced ability to integrate

The amount of reagent and vector in each tube is optimized for lentivirus production in a 10-cm dish. Transfections can be carried out entirely in the presence of serum. Use of tetracycline-free FBS is critical for achieving high titers with this technology.

  At-A-Glance   Documents   Images & Data


  • Fourth-generation lentiviral packaging system (see video) combines unparalleled convenience with high performance
  • Choice of three types of Lenti-X Packaging Single Shots optimized for VSV-G pseudotyped, ecotropic, or integrase-deficient lentivirus
  • Individual tubes contain lyophilized transfection reagents (Xfect polymer and buffer) and lentivirus packaging plasmids (5-component mix combined in an optimized ratio)
  • Consistently high titers (107–108 IFU/ml) no matter which lentiviral source vector you choose
  • Simple protocol—Just add your lentiviral DNA, mix, and add to 293T cells
  • No optimization or additional transfection reagent required


  • Lentiviral transduction of a broad range of hard-to-transfect cell types
  • Easily create stable cell lines
  • Create VSV-G pseudotyped, ecotropic, or integrase-deficient lentivirus

New Cat. # Product Contents Size Price License Units Select
631275 Lenti-X™ Packaging Single Shots (VSV-G) 16 Rxns $787.00 License Statements
631276 Lenti-X™ Packaging Single Shots (VSV-G) 96 Rxns $1,792.00 License Statements
631277 Lenti-X™ Packaging Single Shots (Integrase Deficient) 16 Rxns $787.00 License Statements
631278 Lenti-X™ Packaging Single Shots (Ecotropic) 16 Rxns $787.00 License Statements


Recommended Products
Cat. # Product Contents Size Price Units Select
632180 Lenti-X™ 293T Cell Line 1 mL $295.00
631231 Lenti-X™ Concentrator 100 mL $194.00
631243 Lenti-X™ GoStix™ 20 Tests $313.00

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