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Viral Transduction


Products >  Viral_Transduction >  Lentiviral_Packaging >  Integrase_Deficient_Lentivirus

Integrase-Deficient Lentivirus

Lentiviral vectors are widely used to efficiently transduce both dividing and quiescent cells, which makes them powerful tools for gene delivery. Most commercially available lentiviral packaging systems generate lentivirus that integrates efficiently into the target cell genome as a provirus. However, non-integrating lentivirus can be produced through the use of mutations in the integrase protein that minimize proviral integration. The resulting integrase-deficient lentivirus (IDLV) generates circular vector episomes in transduced target cells that are gradually lost by dilution in dividing cells (transient expression), but are stable in quiescent cells. Inherently, IDLVs have a greatly reduced risk of causing insertional mutagenesis compared to integrating lentiviruses.

The Lenti-X HTX Packaging System (Integrase Deficient) is the only 4th generation lentiviral packaging system for IDLV generation. The system contains a combination of five plasmids mixed in optimal proportions to provide the highest possible lentiviral titers when transfected into Lenti-X 293T cells. The Xfect Transfection Reagent and Tet System Approved FBS are required for the protocol, and are included in the system. Additionally, a sample of Lenti-X GoStix is provided to enable rapid qualitative determination of lentiviral titer.

At-A-Glance Documents Images & Data Resources

Features

  • Transient gene expression in proliferating cells, stable expression in nondividing cells
  • VSV-G pseudotype for broadest host range
  • Highly efficient gene delivery, without insertion into the host genome
  • Reduced risk of insertional mutagenesis
  • Use in combination with any lentiviral vector to generate non-integrative lentivirus
  • Produces high-titer lentivirus

Applications

  • Published uses for IDLV include RNA interference, homologous recombination, site-specific recombination, transposition and iPS cell generation

Additional Product Information

Please see the product's Certificate of Analysis for information aboutstorage conditions, product components, and technical specifications. Pleasesee the Kit Components List to determine kit components. Certificates ofAnalysis and Kit Components Lists are located under the Documentstab.


 
 
Products
Cat. # Product Package Size Price License # of Units Select
631258 Lenti-X™ HTX Packaging System (Integrase Deficient) 20 Rxns $876.00 License Statements
 

 

Required Products
Cat. # Product Package Size Price # of Units Select
632180 Lenti-X™ 293T Cell Line 1 mL $295.00
 

 

Recommended Products
Cat. # Product Package Size Price # of Units Select
631232 Lenti-X™ Concentrator 500 mL $684.00
631243 Lenti-X™ GoStix™ 20 Tests $299.00
631247 Lenti-X™ HTX Packaging System 20 Rxns $876.00
 
 


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