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Viral Transduction


Products >  Viral_Transduction >  Lentiviral_Packaging >  High-Titer_Lentivirus

Lentiviral Packaging Systems

Lenti-X high-titer lentiviral packaging systems generate lentiviral titers that are superior to most other commercially available lentiviral packaging systems. At titers of 107-108 infectious units (IFU) per ml, you can often transduce an entire dish of target cells using only 10–100 µl of unconcentrated lentiviral packaging cell supernatant.

  • No need to concentrate virus
  • No need to transfect multiple plates
  • No need to infect your target cells multiple times
  • Easily control the expression level of your gene of interest by manipulating the multiplicity of infection (MOI)

Lentiviral Packaging Systems—Two Options

The Lenti-X HTX Packaging System is available in two pseudotyping options:

  1. VSV-G lentiviral packaging—The standard and most popular Lenti-X HTX Packaging System produces VSV-G pseudotyped lentivirus, which readily infects virtually all types of cells.
  2. Ecotropic lentiviral packaging—The Lenti-X HTX Ecotropic Packaging System produces lentivirus pseudotyped with the MLV ecotropic envelope glycoprotein, which allows you to limit transduction to mouse and rat cells.

How Are High Lentiviral Titers Achieved?

  • Optimized composition—Our novel 4th Generation Lentiviral Packaging Mixes provide multiple lentiviral packaging and nonviral components in a proprietary suite of 5 vectors that are premixed in specific, ideal ratios in order to maximize virus production. Multiple vectors also effectively prevent the emergence of replication competent viruses.
  • Tetracycline transactivation—Very strong expression of several key lentiviral packaging components is produced by Tet-Off transactivation of tetracycline-responsive promoter elements (TREs). In many cell lines, including HEK 293 cells, the tetracycline transactivator generates absolute expression levels much higher than those produced by the CMV promoter.
  • Optimized transfections—Our exceptional, nanoparticle-based transfection reagent, Xfect, is included with every lentiviral packaging system. This reagent consistently transfects Lenti-X 293T cells with very high efficiencies (> 95%) and allows high expression of viral components.
  • Simple monitoring of lentivirus production—Lenti-X GoStix (included) let you instantly determine the optimal time to harvest your lentiviral supernatant.

The concerted effects of these advanced components allow Lenti-X 293T Cells (sold separately) to produce the highest amounts of safe, replication-incompetent lentivirus from any of our lentiviral vectors, or any other lentiviral vector.

The viruses are perfect for transducing delicate, hard-to-transfect cell types, such as primary neuroprogenitor cells. The standard Lenti-X HTX Packaging System is supplied with many of our lentiviral expression systems. Visit our lentiviral vectors page to see the variety of vectors and systems available.

At-A-Glance Documents Images & Data Resources

Features

  • Generates up to 25 times more lentivirus than other popular systems
  • Obtain >108 IFU/ml from one 10 cm plate
  • Very high safety profile, 5 vector split packaging technology and inducible promoters (4th Generation)
  • Compatible with any lentiviral vector system
  • Create lentiviral supernatants that have VSV-G or Ecotropic envelopes, or integrase deficient lentivirus (IDLV)

Applications

  • Lentiviral transduction of a broad range of hard-to-transfect cell types
  • Easily create stable cell lines
  • Create ecoptropic lentivirus
  • Create VSV-G pseudotyped lentivirus

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Package Size Price License # of Units Select
632180 Lenti-X™ 293T Cell Line 1 mL $295.00  
631251 Lenti-X™ HTX Ecotropic Packaging System 20 Rxns $876.00 License Statements
631247 Lenti-X™ HTX Packaging System 20 Rxns $876.00 License Statements
631249 Lenti-X™ HTX Packaging System 60 Rxns $1,792.00 License Statements
 

 

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