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Viral Transduction


Products >  Viral_Transduction >  Lentiviral_Packaging >  Ecotropic_Lentivirus

Ecotropic Lentivirus

The Lenti-X HTX Ecotropic Packaging System produces high-titer lentivirus pseudotyped with the mCAT-1 envelope glycoprotein, which allows you to limit transduction to mouse and rat cells. It is also possible to transduce human cell types with ecoptropic lentivirus—but only if you pre-treat those cells with Ecotropic Receptor Booster, which allows for a high infection efficiency regardless of the cell line used.

Lenti-X HTX Ecotropic Packaging System

The Lenti-X HTX Ecotropic Packaging System produces lentivirus pseudotyped with the MLV ecotropic envelope glycoprotein, which allows you to limit transduction to mouse and rat cells. The system uses a 4th generation lentiviral packaging technology resulting in high expression of the viral components when transfected into the Lenti-X 293T Cell Line (sold separately). The kit contains the following components:

  • Lenti-X HTX Ecotropic Packaging Mix 2
  • Xfect Transfection Reagent (Cat. No. 631317)
  • Tet System Approved FBS, US Sourced (Cat. No. 631105)
  • Lenti-X GoStix (Sample)

Ecotropic Receptor Booster

The Ecotropic Receptor Booster is used to temporarily elevate the density of the ecotropic receptor protein mCAT-1 on the surface of any target cell that you wish to infect. For example, this technology can be used to efficiently transduce human cells with ecotropic retrovirus or lentivirus that otherwise could only infect rodent cells. The technology also allows you to increase the infection efficiency of any type of cells that are resistant to viral infection, generating a high transfection efficiency regardless of the cell type used.

At-A-Glance Documents Images & Data Resources

Features

  • The Lenti-X HTX Ecotropic Packaging System allows you to limit transduction to mouse and rat cells
  • 4th Generation Lentiviral Packaging System; transactivation cascade results in high expression of the viral components in Lenti-X 293T cells, leading to high lentivirus titers
  • Ecotropic Receptor Booster increases the efficiency of transduction by temporarily increasing the concentration of viral receptor proteins on your target cells, and even permits efficient transduction of human cells
  • Applying the Ecotropic Receptor Booster to your target cells leads to high infection efficiency, regardless of the cell line used

Applications

  • Lentiviral transduction of mouse and rat cells
  • Lentiviral transduction of human cells, if the Ecotropic Receptor Booster is used

Additional Product Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
New Cat. # Product Package Size Price License # of Units Select
  631251 Lenti-X™ HTX Ecotropic Packaging System 20 Rxns $876.00 License Statements
631471 Ecotropic Receptor Booster 20 Rxns $245.00 License Statements
 

 

Recommended Products
Cat. # Product Package Size Price # of Units Select
632180 Lenti-X™ 293T Cell Line 1 mL $295.00
631231 Lenti-X™ Concentrator 100 mL $194.00
631243 Lenti-X™ GoStix™ 20 Tests $299.00
 
 


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