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Viral Transduction


Products >  Viral_Transduction >  Hematopoietic_Cell_Transduction >  T_Cell_Media

GT-T551 Medium for T-Cell Culture and Expansion

GT-T551 is a culture medium ideally suited for expansion of human T lymphocytes (T cells). It is supplemented with human albumin (pharmaceutical grade), recombinant human insulin, L-glutamine, and streptomycin; no additional proteins or growth factors are added. The medium can support stable growth in the presence or absence of serum.

Anti-CD3/IL-2 T-cell activation can also be performed in this medium (Wang et al. 2013; Ai et al. 2014; Dodo et al. 2014). When using this activation method, the additional presence of RetroNectin enhances the proliferation of T cells (Yu et al. 2008; Ishikawa et al. 2014; Hosoi et al. 2014; Sakamoto et al. 2015; Li et al. 2015) and results in an expanded cell population that shows a higher proportion of naïve T cells than anti-CD3 antibody stimulation alone (Hosoi et al. 2014).

  At-A-Glance   Documents   Images & Data

Features

  • Used for extended culture or expansion of T cells
  • Supplemented with human serum albumin, human insulin, L-glutamine, and streptomycin
  • Endotoxin tested (endotoxin <0.3 EU/ml)
  • Compatible with CultiLife Culture Bags for large-scale expansion of T cells
  • Anti-CD3/IL-2 T-cell activation in the presence of RetroNectin enhances proliferation and leads to a higher proportion of naïve T cells. For more information, the user manual can be found under the Documents tab.
  • The method of T-cell expansion by RetroNectin stimulation requires a license from Takara Bio Inc. for uses other than research. Contact bio-licensing@takara-bio.co.jp for details.

Applications

  • Expansion of T cells
  • Gene transfer by lentiviral or retroviral transduction

Storage

Store culture medium at 4°C.

References

Ai, Y. Q. et al. The clinical effects of dendritic cell vaccines combined with cytokine-induced killer cells intraperitoneal injected on patients with malignant ascites. Int. J. Clin. Exp. Med. 7, 4272–81 (2014).

Dodo, K. et al. An efficient large-scale retroviral transduction method involving preloading the vector into a RetroNectin-coated bag with low-temperature shaking. PLoS One 9, e86275 (2014).

Hosoi, H. et al. Stimulation through very late antigen-4 and -5 improves the multifunctionality and memory formation of CD8+ T cells. Eur. J. Immunol. 44, 1747–58 (2014).

Ishikawa, T. et al. Phase I clinical trial of fibronectin CH296-stimulated T cell therapy in patients with advanced cancer. PLoS One 9, e83786 (2014).

Li, W. et al. Efficacy of RetroNectin-activated cytokine-induced killer cell therapy in metastatic brain tumor patients. Oncol. Res. Treat. 38, 160–5 (2015).

Sakamoto, N. et al. Phase I clinical trial of autologous NK cell therapy using novel expansion method in patients with advanced digestive cancer. J. Transl. Med. 13, 277 (2015).

Wang, Y. et al. CIK cells from recurrent or refractory AML patients can be efficiently expanded in vitro and used for reduction of leukemic blasts in vivo. Exp. Hematol. 41, 241–52.e3 (2013).

Yu, S. S. et al. In vivo persistence of genetically modified T cells generated ex vivo using the fibronectin CH296 stimulation method. Cancer Gene Ther. 15, 508–16 (2008).

Additional Product Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
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