Clontech
login | register | order now | view cart | feedback
Products
Viral Transduction


Products >  Viral_Transduction >  Hematopoietic_Cell_Transduction >  RetroNectin_for_Hematopoeitic_Cells

TECH NOTE

Efficient Retrovirus- or Lentivirus-Mediated Gene Transfer to Hematopoietic Cells

RetroNectin Reagent

Overview

Most hematopoietic cells, including hematopoietic stem cells (HSCs) and terminally differentiated cells (e.g., primary T cells and macrophages), are refractory to viral gene delivery. Inefficient transduction of HSCs is a significant obstacle to research into gene therapy strategies for hematopoietic diseases.

The efficiency of lentiviral and retroviral infection of hematopoietic cells is strongly dependent on various factors, including the cell cycle status of the target cells and cell surface receptor expression. To enhance binding and entry of viruses, cofactors are commonly added during transduction of hematopoietic cells. Cationic polymers (e.g., Polybrene), cationic lipids (e.g., Lipofectin or Lipofectamine), and cationic peptides (e.g., protamine) are used as transduction enhancers that neutralize membrane charge and/or promote virus aggregation. However, many of these additives are highly cytotoxic and often negatively impact cell viability and/or proliferation.

Enhanced Virus-Cell Co-localization

The RetroNectin polypeptide consists of three functional domains derived from human fibronectin protein. RetroNectin reagent enhances transduction by facilitating co-localization of viral particles and hematopoietic cells—virus particles bind to the RetroNectin peptide via interaction with the heparin-binding domain, and hematopoietic cells bind mainly through the interaction of the cell surface integrin receptor VLA-4 with the CS-1 site.

Learn more about how RetroNectin reagent works >>

RetroNectin reagent mechanism

The hypothesized mechanism of RetroNectin-mediated enhancement of transduction. The cells interact with the RetroNectin molecule via cell surface receptors, and lentiviral and retroviral particles bind to the H-domain of RetroNectin. These interactions increase the localized concentrations of cells and viral particles, an effect that enhances gene transduction.

High-Efficiency Transduction of Hematopoeitic Cells

By facilitating close physical proximity of cells and viral particles, RetroNectin reagent can enhance viral-mediated gene transfer to a variety of hematopoietic cell types.

Gene transfer efficiency for hematopoietic cells
Cell type Efficiency (%)
Human CD34+ CD38– BMC2 95.5
Human PBMC3 91.2
TF-1 97.9
SupT1 97.3
Jurkat 80.1
K-562 90.4
HL-60 86.1
Monkey CD34+ BMC 72.0
Monkey CD4+ T-cell 85.0
1 Transductions were performed using the RetroNectin-Bound Virus (RBV) Method as described in the User Manual.
2 Bone marrow cells.
3 Peripheral blood mononuclear cells.

Improved Transduction Compared with Conventional Methods

RetroNectin reagent provides better retroviral and lentiviral transduction efficiency for hard-to-infect cell types (e.g., hematopoietic cells) than conventional transduction protocols, including those that use Polybrene. For human CD34+ cells, RetroNectin reagent can increase transduction efficiency by 50–70% (see example in Figure 1). In addition to improved transduction, virtually no cell toxicity is observed in reactions that use RetroNectin reagent.

Gene transfer efficiency with RetroNectin reagent or Polybrene

Figure 1. Comparison of RetroNectin reagent and Polybrene for retroviral transduction of human HSCs. The efficiency of gene transfer was compared when using either a conventional Polybrene method or RetroNectin reagent for retroviral delivery of GFP to human CD34+ HSCs.Fluorescence was measured by flow cytometry after transduction. GFP expression is plotted on the x-axis of the histograms, and the percentage of CD34+ cells that express GFP is shown on the plot. Transduction efficiency was 86-fold higher when RetroNectin reagent was used than when Polybrene was used (25.8% and 0.3%, respectively).

Summary

Research using hematopoietic cells has been limited in part by low efficiency of gene transfer. RetroNectin reagent promotes co-localization of lentivirus or retrovirus with hematopoietic cells to dramatically enhance transduction efficiency.

Methods

The GFP gene was inserted into the BamHI site of the pDON-AI vector (pDON-AI-GFP). This plasmid was transfected into packaging cells to generate recombinant retroviral particles. For transduction, CD34+ HSCs were pre-stimulated with SCF, IL-3, and IL-6. CD34+ cells were infected with the prepared recombinant retrovirus (DON-AI-GFP) either in RetroNectin reagent-coated plates or in the presence of Polybrene. After 48 hours, the percentage of cells expressing GFP was determined using flow cytometry.

Purchasing information and documents >>

Learn more about RetroNectin reagent >>

Top of page

Takara Bio USA is a Takara Bio Company © 2017 Privacy Policy | Terms & Conditions | Terms of Use