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Viral Transduction

Products >  Viral_Transduction >  Hematopoietic_Cell_Transduction >  RetroNectin_Reagent

RetroNectin Reagent—Recombinant Human Fibronectin

Research using hematopoietic cells and other suspension cells has been limited in part by low efficiency of gene transfer (transduction and transfection) into these cell types. RetroNectin reagent promotes co-localization of lentivirus or retrovirus with target cells to dramatically enhance transduction efficiency. As a consequence, RetroNectin reagent is now the gold standard transduction enhancer for retroviral/lentiviral gene transfer to hematopoietic cells and holds great promise in clinical application, having been used in over 40 clinical trials.

What is RetroNectin?

RetroNectin reagent is a recombinant human fibronectin fragment (rFN-CH-296) that contains three functional domains: the cell-binding domain, the heparin-binding domain, and the CS-1 sequence.

How does RetroNectin work?

RetroNectin enhances lentiviral- and retroviral-mediated gene transduction by aiding the co-localization of target cells and viral particles. Specifically, virus particles bind RetroNectin reagent via interaction with the H-domain, and target cells bind mainly through the interaction of cell surface integrin receptor VLA-5 and VLA-4 with the fibronectin C-domain and CS-1 site, respectively. By facilitating close physical proximity, RetroNectin reagent can enhance viral-mediated gene transfer to target cells expressing integrin receptors VLA-4 and/or VLA-5. VL-4-expressing cells include T cells, B cells, monocytes, NK cells, eosinophils, bone marrow monocytic cells, and lymphoid progenitors. Thymocytes, activated T-cells, and mast cell express VLA-5.

RetroNectin reagent also can be used to enhance T cell expansion in vitro.

T lymphocyte expansion from peripheral blood mononuclear cells (PBMCs) is usually performed in the presence of interleukin-2 (IL-2) and anti-CD3 antibody stimulation. The efficiency of T cell expansion can be significantly increased in the presence of RetroNectin reagent. Moreover, the T cell population after expansion in the presence of RetroNectin reagent contains a high proportion of naive T cells.

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Notice to customers: the lyophilized format of RetroNectin GMP grade (T201, 2.5 mg) is being phased out and replaced with a liquid format of Retronectin GMP grade (T202; 2.5 mg at 1 mg/ml in 12.5 mM sodium citrate with 1.25% sucrose, pH 6.2).


  • Enhanced gene transfer efficiency for retroviral or lentiviral transduction of suspension cells, hematopoietic stem cells, and other hard-to-infect cell types
  • Less toxicity than other commonly used transduction-enhancers, including polybrene and protamine
  • Fully recombinant peptide
  • Cat. No. T110A consists of ten 35 mm dishes pre-coated with RetroNectin
  • RetroNectin GMP grade can be used for ex vivo clinical applications for investigational use only; for GMP grade, please complete and return this form prior to purchase
  • The method of T-cell expansion by RetroNectin stimulation requires a license from Takara Bio Inc. for uses other than research. Contact for details


  • Retroviral or lentiviral transduction of cells that express VLA-4 or VLA-5
  • Cell migration assays
  • In vitro T cell expansion
  • Gene therapy studies—RetroNectin reagent has been used in over 40 clinical trials


Research Grade RetroNectin (Cat. No. T100A/B; T110A): For research use only. Not for use in diagnostic or therapeutic procedures.

RetroNectin GMP grade (Cat. Nos. T201, T202): Manufactured as a quality-assured product, according to guidelines for Good Manufacturing Practice (GMP) for Investigational Products. This product can be used for ex vivo clinical applications and is limited to investigational use only.

FAQs Regarding Proper Use of RetroNectin GMP grade

  • Question: Can I use Takara Bio's RetroNectin GMP grade in clinical trials?

Answer: Researchers are free to use this product for development of research-based clinical trials. Proper regulatory approval is required before using the GMP-grade product in research-based clinical trials since these trials may require special considerations or more stringent manufacturing standards.

  • Question: Can I use Takara Bio's RetroNectin GMP grade for commercial use?

Answer: Researchers are free to use the product in accordance with the Notice to Purchaser, but commercial use is not allowed under our general Terms and Conditions for purchase of these products. Commercial access to the GMP-grade product may be available through a formal License and Supply Agreement. In all cases, it is the researcher’s responsibility to get approval from the appropriate regulatory authorities for the intended use of these ancillary materials for your application.

  • Question: Can I use RetroNectin GMP grade for in vivo clinical use?

Answer: No. The product cannot be used for direct in vivo applications. The product is intended for use as an ancillary reagent in a manufacturing process which includes other steps prior to any in vivo application.

  • Question: Is RetroNectin GMP grade (#T202) registered in DMF?

Answer: The liquid format Retronectin GMP grade is in the process of being registered.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the product User Manual for protocols. Certificates of Analysis and User Manuals are located under the Documents tab.

Cat. # Product Contents Size Price License Units Select
T100B RetroNectin® Recombinant Human Fibronectin Fragment 2.5 mg $658.00 License Statements
T110A RetroNectin® Precoated Dish 10 Dishes $338.00  
T100A RetroNectin® Recombinant Human Fibronectin Fragment 0.5 mg $170.00 License Statements
T201 RetroNectin® GMP grade 2.5 mg $1,442.00 License Statements
T202 RetroNectin® GMP grade 2.5 mL $1,442.00 License Statements


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