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Products >  Viral_Transduction >  Hematopoietic_Cell_Transduction >  RetroNectin_Reagent

RetroNectin Reagent—Recombinant Human Fibronectin

Research using hematopoietic cells and other suspension cells has been limited in part by low efficiency of gene transfer (transduction and transfection) into these cell types. RetroNectin reagent promotes co-localization of lentivirus or retrovirus with target cells to dramatically enhance transduction efficiency. As a consequence, RetroNectin reagent is now the gold standard transduction enhancer for retroviral/lentiviral gene transfer to hematopoietic cells and holds great promise in clinical application, having been used in over 40 clinical trials.

What is RetroNectin?

RetroNectin reagent is a recombinant human fibronectin fragment (rFN-CH-296) that contains three functional domains: the cell-binding domain, the heparin-binding domain, and the CS-1 sequence.

How does RetroNectin work?

RetroNectin enhances lentiviral- and retroviral-mediated gene transduction by aiding the co-localization of target cells and viral particles. Specifically, virus particles bind RetroNectin reagent via interaction with the H-domain, and target cells bind mainly through the interaction of cell surface integrin receptor VLA-5 and VLA-4 with the fibronectin C-domain and CS-1 site, respectively. By facilitating close physical proximity, RetroNectin reagent can enhance viral-mediated gene transfer to target cells expressing integrin receptors VLA-4 and/or VLA-5. VL-4-expressing cells include T cells, B cells, monocytes, NK cells, eosinophils, bone marrow monocytic cells, and lymphoid progenitors. Thymocytes, activated T-cells, and mast cell express VLA-5.

RetroNectin reagent also can be used to enhance T cell expansion in vitro.

T lymphocyte expansion from peripheral blood mononuclear cells (PBMCs) is usually performed in the presence of interleukin-2 (IL-2) and anti-CD3 antibody stimulation. The efficiency of T cell expansion can be significantly increased in the presence of RetroNectin reagent. Moreover, the T cell population after expansion in the presence of RetroNectin reagent contains a high proportion of naive T cells.

  At-A-Glance   Documents   Images & Data   Resources

Features

  • Enhanced gene transfer efficiency for retroviral or lentiviral transduction of suspension cells, hematopoietic stem cells, and other hard-to-infect cell types
  • Less toxicity than other commonly used transduction-enhancers, including polybrene and protamine
  • Fully recombinant peptide
  • Clinical grade RetroNectin (GMP) can be used for ex vivo clinical applications (investigational use only) - for GMP grade only please complete and return this form prior to purchase

Applications

  • Retroviral or lentiviral transduction of cells that express VLA-4 or VLA-5
  • Cell migration assays
  • In vitro T cell expansion
  • Growth substrate for stem cells (e.g., induced pluripotent stem cells, iPSCs; embryonic stem cells, ESCs)
  • Gene therapy studies—RetroNectin reagent has been used in over 40 clinical trials

Notes

Research Grade RetroNectin (Cat. No. T100A/B; T110A): For research use only. Not for use in diagnostic or therapeutic procedures.

Clinical Grade RetroNectin (GMP) (Cat. No. T201): RetroNectin (GMP) is manufactured as a quality-assured product, according to guidelines for Good Manufacturing Practice (GMP) for Investigational Products. This product can be used for ex vivo clinical applications and is limited to investigational use only.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the product User Manual for protocols. Certificates of Analysis and User Manuals are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
T100B RetroNectin® Recombinant Human Fibronectin Fragment 2.5 mg $645.00 License Statements
T110A RetroNectin® Precoated Dish 10 Dishes $331.00 License Statements
T100A RetroNectin® Recombinant Human Fibronectin Fragment 0.5 mg $166.00 License Statements
T201 RetroNectin® (GMP) 2.5 mg $1,400.00 License Statements
 

 

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