Tetracycline-inducible expression of lacZ using adenovirus.
The Adeno-X Tet-On 3G Systems are highly inducible. Using equal amounts of high-titer supernatants, HeLa cells cultured at the indicated concentrations of Dox were infected with Adeno-X Tet-On 3G LacZ virus. Cultures were harvested and assayed for beta-galactosidase activity using the Luminescent Beta-gal Reporter System 3 (Cat. No. 631713).