Purify adenovirus quickly and easily with our adenovirus purification kits! These kits are chromatography-based systems that enable you to purify amplified adenovirus directly from the cell pellet in less than 1.5 hours. You simply pellet the cells when the cytopathic effect (CPE) is nearly complete, lyse the cells, and purify the virus.
Our Adeno-X Maxi Purification Kits have a wide range of viral capacities, allowing you to scale up or down without difficulty. The kit delivers as many as 1 x 1012 adenoviral particles, and provides all of the buffers and materials necessary for purification. This same technology is also available in our convenient Adeno-X Mega Purification Kits with which you can produce and purify up to 1013 highly infectious viral particles (up to 3 x 1011 IFU).
Unsure Which Adenovirus Purification Kit To Use?
|How much lysate do I have? ||Which kit do I need? |
|5 x 15 cm plates ||Adeno-X Maxi Purification Kit|
|25 x 15 cm plates ||Adeno-X Mega Purification Kit|
Simple Chromatographic Procedure
Our protocol contains no ultracentrifugation steps. Instead, adenovirus is purified chromatographically using a unique membrane that selectively binds adenoviral particles. The membrane is housed in small, single-use cartridge that fits securely on standard size Luer-Lok syringes. For added convenience, the syringe-filter assembly comes completely preassembled and ready to use. The adenovirus-containing medium is brought into the syringe through tubing attached to a one-way valve, and is pushed out through the purification cartridge. As virus-containing medium passes through the purification cartridge, the adenoviral particles adhere to the membrane and are effectively removed from the solution. The bound particles are then washed and eluted.
Achieve High Titers of Purified Adenovirus
The high titer and purity achieved by our Adeno-X Purification Kits is comparable to that achieved by CsCl density gradient centrifugation, with viral particle to infectious unit ratios as low as 20:1. Unlike techniques involving CsCl, our method is safe, and requires no advanced training or expensive equipment—only simple manipulations are required to produce consistently high yields of purified adenovirus.
A Powerful Pump Makes Scaling Up Rapid and Easy
To collect large amounts of virus use the Mega kit. With this kit we have adopted the use of a GENIE Plus syringe pump to drive the clarified lysate through the 3-tiered purification cartridge. The consistent pressure and flow rate provided by the pump during the binding, wash and elution steps provides excellent virus recovery.