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Products >  Viral_Transduction >  AAV_Vector_Systems >  AAV_Tet_Inducible_Expression_System

Single Vector Tetracycline Inducible Expression with AAV

Tet-One Systems provide all necessary components for tetracycline (tet) inducible expression on a single vector. The Tet-On 3G transactivator is expressed in from the human phosphoglycerate kinase 1 (PGK) promoter, and the cloned gene of interest is expressed from the PTRE3GS promoter. This all-in-one design has been shown to result in up to 25,000-fold induction (Heinz, N. et al., 2011).

There are several options for preparing and delivering Tet-One vectors to mammalian cells. The AAVpro Tet-One Inducible Expression System allows production of high titers of recombinant, adeno-associated virus (AAV) to establish tet-inducible expression of your gene of interest. The AAVpro system contains the reagents to generate the Tet-One vector, as well as components to efficiently package and isolate AAV2 particles. This kit uses helper-free packaging, a unique system for preparing high-titer AAV2 particles without the use of a helper virus.

  At-A-Glance   Documents   Images & Data

Features

  • All-in-one vector design allows you to establish tet-inducible expression of your gene of interest with a single vector
  • Low background expression and high induction
  • Transactivator expression from the human PGK promoter allows inducible expression in a broad range of tissues
  • The unique AAV packaging system allows preparation of high-titer AAV2 without the use of a helper virus
  • Kit includes AAV extraction solutions that increase viral particle recovery as compared to conventional freeze-thaw methods

Applications

  • Tightly-controlled, tetracycline-inducible expression of your gene of interest in mammalian cells

References

Heinz, N. et al. (2011) Retroviral and transposon-based Tet-regulated all-in-one vectors with reduced background expression and improved dynamic range. Human Gene Ther. 22(2):166–176.

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
634310 AAVpro® Tet-One™ Inducible Expression System (AAV2) 1 System $976.00 License Statements *
 
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

 

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