Helper-Virus Free Preparation of AAV-LacZ Particles
The AAVpro Helper Free System (AAV2-LacZ) is a kit to prepare serotype 2 adeno-associated virus (AAV2) particles for delivery of the lacZ reporter gene to mammalian cells. High-titer AAV2 can be prepared without a helper virus by transfecting HEK 293T cells with the three plasmids included in this kit. The plasmids encode all of the factors necessary for viral packaging. After packaging, AAV Extraction Solution is used for efficient AAV2 particle isolation without ultracentrifugation.