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Products >  Viral_Transduction >  AAV_Vector_Systems >  AAV-LacZ

Helper-Virus Free Preparation of AAV-LacZ Particles

The AAVpro Helper Free System (AAV2-LacZ) is a kit to prepare serotype 2 adeno-associated virus (AAV2) particles for delivery of the lacZ reporter gene to mammalian cells. High-titer AAV2 can be prepared without a helper virus by transfecting HEK 293T cells with the three plasmids included in this kit. The plasmids encode all of the factors necessary for viral packaging. After packaging, AAV Extraction Solution is used for efficient AAV2 particle isolation without ultracentrifugation.

  At-A-Glance   Documents   Images & Data

Features

  • High-titer AAV2 particles encoding the lacZ reporter gene under the control of the CMV promoter can be prepared without a helper virus
  • Enables delivery of the lacZ reporter gene to hard-to-transfect mammalian cells, including proliferating and non-proliferating cells
  • The lacZ gene encodes the enzyme beta-galactosidase, a commonly used reporter
  • Expression can be detected with the Beta-Galactosidase Staining Kit

Applications

  • Reporter that allows transduced cells to be identified by immunostaining with an anti-beta-galactosidase antibody or by enzyme activity assay (blue color development in the presence of X-gal)
  • Can be used as a positive control during AAV particle preparation using the AAVpro Helper Free System

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

 
 
Products
New Cat. # Product Contents Size Price License Units Select
  6655 AAVpro® Helper Free System (AAV2-LacZ) 20 ug each $894.00 License Statements
 

 

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