Helper Virus-Free Preparation of AAV Particles to Deliver Cre Recombinase
The AAVpro Helper Free System (AAV2-CRE Recombinase) is a kit for the preparation of serotype 2 adeno-associated virus (AAV2) particles to deliver a Cre recombinase gene cassette to mammalian cells. Cre recombinase is a bacterial protein that recognizes a specific 34-bp sequence (loxP site) containing an 8-bp core sequence where recombination takes place. The orientation and location of introduced loxP sites allow for Cre-mediated gene inversion, translocation, or deletion. With this kit, high-titer AAV2 particles can be prepared without a helper virus by simply transfecting HEK 293T cells with the three included plasmids that encode all of the factors necessary to prepare recombinant AAV particles. AAV Extraction Solution is also included for efficient AAV2 particle isolation.