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Products >  Viral_Transduction >  AAV_Vector_Systems >  AAV-Cre_Recombinase

Helper Virus-Free Preparation of AAV Particles to Deliver Cre Recombinase

The AAVpro Helper Free System (AAV2-CRE Recombinase) is a kit for the preparation of serotype 2 adeno-associated virus (AAV2) particles to deliver a Cre recombinase gene cassette to mammalian cells. Cre recombinase is a bacterial protein that recognizes a specific 34-bp sequence (loxP site) containing an 8-bp core sequence where recombination takes place. The orientation and location of introduced loxP sites allow for Cre-mediated gene inversion, translocation, or deletion.  With this kit, high-titer AAV2 particles can be prepared without a helper virus by simply transfecting HEK 293T cells with the three included plasmids that encode all of the factors necessary to prepare recombinant AAV particles. AAV Extraction Solution is also included for efficient AAV2 particle isolation.

  At-A-Glance   Documents   Images & Data

Features

  • Helper virus-free preparation of high-titer AAV2 particles encoding the Cre recombinase gene under the control of the CMV promoter
  • Enables delivery of the Cre recombinase gene to hard-to-transfect mammalian cells, including proliferating and non-proliferating cells 
  • Cre recombinase mediates recombination between loxP sites, allowing gene inversion, translocation, or deletion 

Applications

  • Genome editing—Transduction of target cells containing a floxed DNA sequence with the AAV2-CRE Recombinase particles results in recombination between the loxP sites
  • Generating transgenic mice

Additional Product Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. The Certificate of Analysis is located under the Documents tab

 
 
Products
New Cat. # Product Contents Size Price License Units Select
  6652 AAVpro® Helper Free System (AAV2-CRE Recombinase) 20 ug each $894.00 License Statements
 

 

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