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Products >  Viral_Transduction >  AAV_Transduction_Tools >  Purification_Kit

Purification of Adeno-Associated Viral Particles from Virus-Producing Cells: AAVpro Purification Kits

The purity of adeno-associated virus (AAV) particles is important for achieving stable and effective transduction into individual animals and cultured cells. When introducing genes into animals (in vivo transduction), it is necessary to use highly-purified particles that do not contain any residual virus-producing cells or impurities from the culture medium. In addition, when transducing cultured cells, purification of AAV particles eliminates potential negative effects of impurities.

Methods such as cesium chloride concentration gradient ultracentrifugation or iodixanol ultracentrifugation are commonly used to purify AAV particles, but such methods are time-consuming and require careful technique to obtain high yields. The AAVpro Purification Kit (AAV2) uses a column-based purification system to quickly and easily purify AAV serotype 2 (AAV2) particles from virus-producing cells. Use this kit after virus preparation with the AAVpro Helper Free System (AAV2) to generate high yields of highly pure, transduction ready AAV2 particles.

The AAVpro Purification Kit (All Serotypes) uses a precipitation and filtration protocol to purify AAV particles of any serotype.

  At-A-Glance   Documents   Images & Data

Features

  • Unique AAV vector extraction methods that eliminate the need for ultracentrifugation
  • Kits include all reagents needed to purify AAV particles from vector-producing cells
  • Purification yields highly pure AAV particles that can be used directly for in vivo transduction or transduction of cultured cells
  • AAVpro Purification Kit (AAV2) is a two-prep kit and does not include Cryonase Cold-Active Nuclease
  • AAVpro Purification Kit (All Serotypes) is a four-prep kit and does include Cryonase Cold-Active Nuclease

Applications

  • AAV particle purification from virus-producing cells

Additional Product Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the User Manual for protocols. Certificates of Analysis and User Manuals are located under the Documents tab.


 
 
Products
Cat. # Product Contents Size Price License Units Select
6232 AAVpro® Purification Kit (AAV2) 2 Preps Call License Statements *
6666 AAVpro® Purification Kit (All Serotypes) 1 Kit $660.00 License Statements
 
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

 

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* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.
 
 
 

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