Improved Method for AAV Particle Extraction from Virus-Producing Cells: AAVpro Extraction Solution
Increase AAV yield by at least 3-fold with AAVpro Extraction Solution, a reagent for the extraction of AAV particles from AAV particle-producing cells. Isolation of AAV particles is typically performed using freeze-thaw or sonication methods. However, these methods are time consuming and require special equipment. The AAVpro Extraction Solution provides an improved method for AAV particle isolation; simply add the Extraction Solutions to virus-producing cells and recover viral particles by centrifugation. In addition to a simple protocol, the Extraction Solution reduces host protein and nucleic acid contamination, yielding viral particle solutions that are ready for cell infection or further purification with the AAVpro Purification Kit*. * For in vivo transduction, purify AAV2 particles with the AAVpro Purification Kit (AAV2), which includes this product, to obtain highly-purified particles that do not contain any residual virus-producing cells or impurities from the culture medium.