AAVpro Packaging Plasmid (AAV2) is a set of two plasmids (pRC-mi342 and pHelper) designed to generate high-titer adeno-associated virus (AAV2). AAV2 particles are generated without the use of a helper virus: simply cotransfect these plasmids and an AAV vector containing your gene of interest into the AAVpro 293T Cell Line (sold separately), and harvest high-titer AAV. The packaging plasmids (pRC2-mi342 and pHelper) are supplied in large quantities (1 mg each), which makes this product suitable for the large-scale AAV production necessary for in vivo studies.
pRC2-mi342 carries the Rep and Cap genes of AAV2 and an expression cassette for a microRNA (hsa-miR-342)*, shown to increase AAV2 titer twofold. *hsa-miR-342 is a human microRNA that increases AAV2 titer in this vector preparation system. It increases titer by approximately twofold as compared to ordinary pRC2 vectors that express only Rep and Cap.
pHelper contains adenovirus E2A, E4, and VA, genes required for efficient AAV production.