Transfection protocol for the Xfect siRNA Transfection Reagent.
Transfection of siRNA using Xfect is very simple. For one well of a 24-well plate, take 30 picomoles of siRNA and 4 µl of Xfect Polymer and suspend each in a total of 50 µl of reaction buffer, then combine and wait 20 min for the nanoparticles to form. Apply to your cells and analyze for knockdown of expression 24–48 hr later. There is no need to test different ratios of polymer to siRNA.