siRNA transfection with Xfect showing viability and siRNA-mediated knockdown data.
Xfect siRNA Transfection Reagent transfects with high efficiency and won't kill your cells. HeLa, 293T, and HT1080 cells plated at 90% confluency in 24-well plates were transfected with 30 pmoles of anti-PP1B (cyclophilin B) siRNA following the recommended protocol. PP1B mRNA levels were analyzed by qPCR after 48 hours of incubation and mRNA levels were normalized against HPRT expression. Cell viabilities were determined after 48 hours of incubation using a dye exclusion assay.