Xfect RNA Transfection Reagent is a complete system for highly efficient transfection of mammalian cells with all types of RNA. Transfection with messenger RNA allows for rapid gene expression, without the risk of genomic integration that comes from transfection with DNA. While most transfection reagents can be harmful to your cells, the Xfect RNA Transfection Reagent has very low cytotoxicity. The reagent can also efficiently deliver single guide RNAs (sgRNA) for CRISPR/Cas9-mediated gene editing. This reagent creates biodegradable nanoparticles when combined with RNA, enabling efficient transfection of mammalian cells without any of the damaging side-effects.
The Xfect RNA Transfection Reagent is compatible with all types of RNA, including mRNA, single guide RNA (sgRNA), microRNA, and siRNA. The protocol is simple, and transfections can be carried out entirely in the presence of serum.
When used in conjunction with the Xfect Transfection Reagent, the Xfect RNA Transfection Reagent can be used for efficient cotransfection of mammalian cells with both DNA and RNA.