The Xfect Protein Transfection Reagent uses a cell-penetrating peptide developed at Clontech to bind and transport active proteins directly into a wide variety of mammalian cell types, including hard-to-transfect human suspension cell lines and mouse embryonic stem cells.
What is the Xfect Protein Transfection Reagent and how does it work?
Xfect Protein Transfection Reagent is a modified peptide with cell-penetrating activity whose amino acid composition enables it to interact with a protein cargo and transport this protein across a cell membrane barrier. Just incubate your protein of interest with Xfect in the supplied buffer for 30 min, then apply the mixture to your cells—and 2 hr later, you are ready to assay for protein activity.
Why transfect purified proteins directly?
Protein transfection is extremely rapid compared to traditional gene expression studies using transfected DNA (1–2 hours compared to 18–48 hours), because it bypasses cellular processes such as transcription and translation. It also facilitates studies involving transient effects of proteins, and avoids potentially harmful, random DNA integration into the genome of the target cells. Xfect Protein Transfection Reagent makes it possible to deliver active proteins directly into cells for studies that involve transcriptional regulation, the cell cycle, apoptosis, oncogenesis, epigenetics, cell regeneration, and transdifferentiation.
What are the advantages of Xfect Transfection Reagent compared to other protein delivery technologies?
The two technologies most frequently used for protein delivery are based on lipids and cell-penetrating peptides. Lipid-based reagents tend to demonstrate high cytotoxicity, while peptide-based reagents transfect with low efficiency. Xfect Protein Transfection Reagent offers the best advantages of both, i.e., it retains low cytotoxicity and delivers more protein to a higher percentage of target cells (see Images & Data tab). Moreover, Xfect Protein can transfect cells that are growing at a higher density than competing products, which is important because assays are often performed within a few hours post-transfection and the higher cell densities ensure sufficient material for downstream analysis.