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Transfection & Cell Culture

Products >  Transfection_and_Cell_Culture >  Product_Overview >  Xfect_Single_Shots >  Visual_Protocol

How to Use Xfect Single Shots

Xfect Single Shots are pre-aliquoted in individual tubes. Just add your DNA sample to perform transfections quickly and easily.

    • Dilute your plasmid DNA
    • Suspend 5 µg of your plasmid containing your gene of interest in 100 µl of water.
    • Add plasmid to Xfect Single Shots tube
    • Add the diluted plasmid to one of the Xfect Single Shots.
    • Vortex the tube for 10 sec
    • Vortex at high speed for 10 sec to mix the sample.
    • Incubate the tube for 10 min
    • Incubate for 10 min at room temperature to allow DNA and Xfect nanoparticle complexes to form.
    • Add plasmid to cells with medium
    • Add the entire 100 µl into a single well of a 6-well plate containing 1 ml of complete growth medium. Peak expression is typically reached 48 hr post-transfection.
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