An obvious way to increase transfection efficiency is to increase the amount of plasmid DNA and transfection reagent mixture that you add to your cells. Unfortunately, since most transfection reagents damage cells during transfection, simply using more of it is not an option. Xfect Transfection Reagent is different! Xfect is a biodegradable transfection polymer, screened from a pool of 2,300 candidates for its very low cytotoxicity profile and high transfection efficiency. Xfect allows you to increase your transfection efficiency to the level you need...without killing your cells. You may also be interested in viewing our Xfect Single Shots Video.
Excellent for Most Cell Types, Even Difficult-to-Transfect Cells
Xfect transfects the following 5 most commonly transfected cell lines with very high efficiency:
| Cell Type | Transfection Efficiency |
| 293T | 97% |
| HEK-293 | 93% |
| HeLa | 85% |
| NIH/3T3 | 82% |
| CHO | 83% |
Xfect also outperforms popular competitor transfection reagents in Jurkat cells, which are notoriously difficult to transfect. Xfect Transfection Reagent produced >40-fold higher transfection efficiency than Product L. This transfection reagent can also be used to transfect human adult stem cells. We used Xfect to transfect human adipose-derived stem cells (hADSCs) with a transfection efficiency of 92%.
Our customers have indicated via survey that they have successfully used Xfect Transfection Reagent with a wide variety of cells and cell lines.
A Simple Protocol
For ease of use, plasmid transfections can be carried out entirely in the presence of serum. The Xfect transfection protocol requires few steps and minimal optimization.
Share Without Worry—Convenient 2-Pack or 3-Pack Format
Xfect Transfection Reagent is packaged in convenient, separable kits, so if you purchase the 100 reaction kit, you can share with your less-than-careful colleagues without fear. Simply give them half of the kit and hide yours! Our 300 rxn kit can be divided into 3 x 100 transfections.
