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Transfection & Cell Culture

Products >  Transfection_and_Cell_Culture >  Antibiotic_Selection_Markers >  Mammalian_Expression_Plasmids

Selectable Mammalian Expression Vectors

The pIRES Selection Vectors pIRESpuro3, pIREShyg3, pIRESneo3, and pIRESbleo3 each contain a multiple cloning site located upstream of a selection marker expressing IRES. Because your gene of interest and a selection marker are translated from a single RNA, you can be certain that nearly 100% of the colonies resistant to puromycin, hygromycin, G418, or bleomycin also express your protein of interest. All IRES Selection Vectors contain a partially disabled IRES which reduces the efficiency of translation initiation for the selection marker relative to that of the cloned gene and allows preferential selection of cells expressing high levels of your protein of interest.

pBApo series of vectors are mammalian expression vectors for constitutive expression of your gene of interest from either a CMV or EF1 alpha promoter. The antibiotic resistance marker, either puromycin or neomycin, is expressed separately from a SV40 promoter. pBApo-CMV lacks a selectable marker.

  At-A-Glance   Documents   Images & Data


  • pIRES vectors include either puromycin, hygromycin, G418, or bleomycin resistance for selection
  • pBApo vectors include either neomycin or puromycin resistance for selection
  • Allow for stable product expression in mammalian cells


  • Express protein-coding genes
  • Express micro RNA precurors and other transcripts
  • pBApo cassette can be cut out and transferred to adenovirus vectors

Additional Product Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Cat. # Product Contents Size Price Units Select
631619 pIRESpuro3 Vector 20 ug $426.00
631620 pIREShyg3 Vector 20 ug $426.00
631621 pIRESneo3 Vector 20 ug $426.00
631622 pIRESbleo3 Vector 20 ug $426.00
3240 pBApo-CMV Neo DNA 20 ug $252.00
3241 pBApo-CMV Pur DNA 20 ug $252.00
3242 pBApo-CMV DNA 20 ug $239.00
3243 pBApo-EF1alpha Neo DNA 20 ug $252.00
3244 pBApo-EF1alpha Pur DNA 20 ug $252.00



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