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Transfection & Cell Culture

Products >  Transfection_and_Cell_Culture >  Antibiotic_Selection >  G418_Puromycin_and_Hygromycin

G418, Puromycin, Hygromycin B

Antibiotics for the selection and maintenance of eukaryotic cells that are stably transfected with vectors expressing neomycin, puromycin, and hygromycin resistance cassettes.

G418, Puromycin & Hygromycin Dose Concentrations for Selection & Maintenance

Recommended Concentration (µg/ml)










Hygromycin B



For selecting colonies, the appropriate dose must be determined empirically for each specific cell line. We recommend to test a dosage range using dishes of untransfected cells and choose the dose that kills all of the cells in 3–5 days. If all the cells die in less than 24 hr, you should use a lower dose.

  At-A-Glance   Documents   Images & Data


  • The most widely used selection agents for transfected mammalian cells carrying the resistance genes NeoR, HygR and PuroR/Pac respectively.
  • G418 Sulfate: An aminoglycoside antibiotic similar to gentamycin. Toxic to bacteria, yeast, higher plant and mammalian cells in addition to protozoans and helminths.
  • Hygromycin B: An aminoglycoside antibiotic that inhibits protein synthesis in bacteria, fungi and higher eukaryotes.
  • Puromycin dihydrochloride: A broad spectrum antibiotic that inhibits protein synthesis in both prokaryotic and eukaryotic organisms.


G418 Sulfate

  • Molecular Weight: 692.7
  • Chemical Formula: C20H40N4O10·2H2SO4
  • Form: White to Off-White Powder


  • Molecular Weight: 544.4
  • Chemical Formula: C22H29N2O5·2HCl
  • Form: White Powder

Hygromycin B

  • Molecular Weight: 527.5
  • Chemical Formula: C20H37N3O13
  • Form: Sterile Brown Solution at 50 mg/ml in PBS

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

Cat. # Product Contents Size Price Units Select
631307 G418 1 g $98.00
631308 G418 5 g $355.00
631309 Hygromycin B 1 g $204.00
631305 Puromycin 25 mg $74.00
631306 Puromycin 100 mg $176.00



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