Antibiotics for the selection and maintenance of eukaryotic cells that are stably transfected with vectors expressing neomycin, puromycin, and hygromycin resistance cassettes.
G418, Puromycin & Hygromycin Dose Concentrations for Selection & Maintenance
| Recommended Concentration (µg/ml) |
| Antibiotic | Selection | Maintenance |
| G418 | 100–800 | 200 |
| Puromycin | 0.25–10 | 0.25 |
| Hygromycin B | 50–400 | 100 |
For selecting colonies, the appropriate dose must be determined empirically for each specific cell line. We recommend to test a dosage range using dishes of untransfected cells and choose the dose that kills all of the cells in 3–5 days. If all the cells die in less than 24 hr, you should use a lower dose.
