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Products >  Stem_Cell_Research >  Services >  Directed_Differentiation

Cellartis Human Pluripotent Stem Cell Services: Directed Differentiation

With Cellartis directed differentiation services, we will deliver high-quality, custom hepatocytes or definitive endoderm cells derived from your human ES or iPS cells. We have over 15 years’ experience differentiating pluripotent cells along the endodermal lineage.

Our differentiation protocols start from pluripotent cells cultured in the Cellartis DEF-CS 500 Culture System, which maintains cells in a highly pluripotent state and ensures a stable karyotype. This ensures high-quality starting material for efficient directed differentiation. As part of our service, cells previously cultured in other systems will be adapted to the DEF-CS culture system prior to differentiation.

For more information, visit the services overview page and fill out the inquiry form on the right-hand side.

  At-A-Glance

Features

Our directed differentiation package to generate hepatocytes or definitive endoderm (DE) cells from your human ES or iPS cells1 includes:

  • Thawing of your cells and adaptation to the Cellartis DEF-CS 500 Culture System
  • Expansion of the cells in the DEF-CS system and generation of a Seed Bank (QC testing included)
  • Differentiation into the final cell product and banking
  • Characterization of the banked, final cell product (QC testing included)

QC testing included with the directed differentiation package:

  • Visual inspection and confirmation of the morphologies of the cells in the starting material and in the final cell product
  • Immunocytochemistry to verify the presence of relevant mature markers and the absence of the pluripotency marker OCT-4 in the final cell product
  • Sterility testing of the final cell product (bacteria and Mycoplasma)
Additional QC testing and characterization services for directed differentiation into hepatocytes:
  • Cellartis Cytochrome P450 Activity Profiling Service (Y60307)
  • Cellartis Hepatic Gene Expression Profiling Service (Y60308)
  • Cellartis Hepatic Protein Expression Profiling Service (Y60309)

1 As an alternative to providing your own cells, you may request Cellartis sourcing, reprogramming, or banking services.

For more information about this and other services, visit the services overview page and fill out the inquiry form on the right-hand side.

Details

Cellartis hiPS Directed Differentiation to Hepatocytes Service (Y60220)

  • Starting materials: 5 vials (2.5 million cells per vial) of customer-provided hiPS cells, with relevant information regarding culturing and freezing protocols media contents
  • Deliverables: project report, 30 vials of hepatocytes (12 million viable cells per vial)
  • Lead time: 20 weeks*

Cellartis hES Directed Differentiation to Hepatocytes Service (Y60221)

  • Starting materials: 5 vials (2.5 million cells per vial) of customer-provided hES cells, with relevant information regarding culturing and freezing protocols
  • Deliverables: project report, 30 vials of hepatocytes (12 million viable cells per vial)
  • Lead time: 20 weeks*

Cellartis hiPS Directed Differentiation to DE Cells Service (Y60210)

  • Starting materials: 5 vials (2.5 million cells per vial) of customer-provided hiPS cells, with relevant information regarding culturing and freezing protocols
  • Deliverables: project report, 30 vials of DE cells (6 million viable cells per vial)
  • Lead time: 14 weeks*

Cellartis hES Directed Differentiation to DE Cells Service (Y60211)

  • Starting materials: 5 vials (2.5 million cells per vial) of customer-provided hES cells, with relevant information regarding culturing and freezing protocols
  • Deliverables: project report, 30 vials of DE cells (6 million viable cells per vial)
  • Lead time: 14 weeks*

* Actual time may vary based on additional customer requirements.

For more information about this and other services, visit the services overview page and fill out the inquiry form on the right hand side.

Notes

For safety and QC purposes, we can only work with human samples that are free of human pathogens and that have passed a sterility test. We require documentation proving that all samples shipped to us are sterile and pathogen-free. In the absence of documentation, testing for pathogens (Y60301) and sterility (Y60302) will be performed via a 3rd party service prior to the initiation of Cellartis services. Please note that safety and sterility testing will incur additional lead time and costs.

Additional Product Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


 
 
Products
Cat. # Product Size Price License Units Select
Y60301 Cellartis® Human Pathogen and Mycoplasma Testing Service Each $825.00  
Y60302 Cellartis® Sterility Testing Service Each $353.00  
Y60210 Cellartis® hiPS Directed Differentiation to DE Cells Service Each Call License Statements
Y60211 Cellartis® hES Directed Differentiation to DE Cells Service Each Call License Statements
Y60220 Cellartis® hiPS Directed Differentiation to Hepatocytes Service Each Call License Statements
Y60221 Cellartis® hES Directed Differentiation to Hepatocytes Service Each Call License Statements
Y60307 Cellartis® Cytochrome P450 Activity Profiling Service Each Call  
Y60308 Cellartis® Hepatic Gene Expression Profiling Service Each Call  
Y60309 Cellartis® Hepatic Protein Expression Profiling Service Each Call  
 

 

 
 

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