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Stem Cell Research

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Stem Cell Webinar Series

We are committed to providing expert scientific support to advance your stem cell research. The following webinars will introduce you to some of our valuable tools that will enable you to perform good science.

Visual Protocol: Editing Human iPSCs with CRISPR/Cas9 and Single-Cell Cloning

Topics include:

  • Step-by-step protocol—generate edited human iPS cell lines using electroporation-based delivery of RNP complexes and single-cell cloning
  • High-efficiency workflow—greatly improve single-cell survival post-seeding using the Cellartis DEF-CS culture system
  • Alternate RNP delivery method—in addition to our electroporation-based kit, we also offer a gesicle-delivery version
  • Start-to-finish editing and single-cell cloning kits—complete systems make human iPS cell editing routine instead of frustrating

Order the Cellartis iPSC rCas9 Electroporation and Single-Cell Cloning System

Footprint-free gene editing using CRISPR/Cas9 and single-cell cloning of edited human iPS cells

Topics include:

  • Introduction to gene editing with pluripotent stem cells—learn about the challenges and downstream applications
  • Gene editing with the Cellartis DEF-CS 500 Culture System—monolayer format enables the survival of edited single cells and the pluripotency of the resulting clonal lines
  • Endogenous CD81 knockout experiments—the DEF-CS system results in successful editing, cloning, expansion, and maintenance of stemness markers

Order the Cellartis iPSC Single-Cell Cloning DEF-CS Culture Media Kit now

Single-Cell Cloning of Human iPS Cells—An Optimized Culture System for Creating Highly Pluripotent, Edited Clonal Lines

Topics include:

  • Introduction to gene editing with pluripotent stem cells—learn about the challenges and downstream applications
  • Single-cell cloning with the Cellartis DEF-CS 500 Culture System—monolayer format improves hiPS cell quality and enables single-cell culture
  • Comparison between the Cellartis system and competitors’ systems for single-cell cloning—the DEF-CS culture system enables the highest survival and pluripotency

Order the Cellartis iPSC Single-Cell Cloning DEF-CS Culture Media Kit now

A Complete Culture System for Human Induced Pluripotent Stem Cells

Topics include:

  • Solutions for maintaining human induced pluripotent stem cell lines—feeder-free, defined conditions and single-cell enzymatic passaging provide unparalleled experimental flexibility
  • DEF-CS culture system as a complete solution to purity issues—promotes homogeneous undifferentiated cells with virtually no spontaneous differentiation
  • Description of the step-by-step protocol—includes passaging, guidelines for cell line transfer from other systems, and applications

Order the Cellartis DEF-CS 500 Culture System now

Insulin-Secreting Human iPSC-Derived Beta Cells for Reproducibly Modeling and Understanding Disease

Topics include:

  • Beta cell characteristics and incretin response—ideal for diabetes research, with expected RNA and protein expression of relevant markers (e.g., insulin, MAFA, and NKX6.1)
  • Suitability for in vitro assays—cells express mature beta-cell markers including insulin, and demonstrate glucose-stimulated insulin secretion (GSIS) after cryopreservation
  • Speeding up your drug-discovery process—we produce functional iPSC-derived cell models, helping you understand disease biology and find better drug candidates

Order the Cellartis hiPS Beta Cells (from ChiPSC12) Kit now

Using human iPSC-derived hepatocytes with advanced functionality and longevity: innovative tools for disease modeling and drug discovery

Topics include:

  • Description of a robust hepatocyte differentiation protocol—recapitulates in vivo liver development and allows differentiation of mature and functional hepatocytes from multiple iPS cell lines
  • Advantages of a novel maintenance medium—significantly improves hepatocyte functions and increases longevity to 19 days post-thawing
  • Applications for metabolic disease modeling and drug development—data gathered over a 14-day assay window shows sustained functionality of drug metabolizing machinery, and metabolic disease-relevant features

Order Cellartis Enhanced hiPS-HEP cells now

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