The iPS Efficiency Check qPCR Kit lets you easily quantitate expression levels of key senescence markers to help you determine somatic cell reprogrammability, senescence marker expression levels (before and during reprogramming), and whether siRNA or knockdown construct(s) are required to enhance the efficiency of induced pluripotent stem (iPS) cell generation.
The kit provides all of the reagents needed to assess your target cell’s reprogrammability. It features prevalidated, forward and reverse primer mixes specific for four key senescence markers and one housekeeping (control) gene (Table I.) It also includes all of the reagents necessary for RNA extraction, first-strand cDNA synthesis, and quantitative real-time PCR. We also offer the iPS Efficiency Check qPCR Primer Set—which simply contains the prevalidated, forward and reverse primer mixes listed in Table I—for easy integration into your lab's existing workflow.
Table I. iPS Efficiency Check qPCR Primers
|Primer Set||Amplicon Size|
|Arf (senescence marker)||67 bp|
|Ink4a (senescence marker)||59 bp|
|p21 (senescence marker)||140 bp|
|p53 (senescence marker)||109 bp|
|Beta-Actin (control)||151 bp|